Perform a DNA extraction from green peas at home or school lab, under adult supervision, by following the given procedure carefully.

Procedure for DNA extraction:

To half cup of split peas, add 1/8 teaspoon of salt and one cup of cold water.
Transfer this mixture in a blender and blend it on high speed for 15 seconds.
Filter the blended mixture, using a strainer, into a measuring container. Observe the amount of mixture you obtain after filtration. Add liquid detergent equal to 1/6 volume of the obtained pea mixture.
Mix by swirling. Allow the mixture to rest for about five to ten minutes.
Transfer this mixture to test tubes. Fill the tubes to about 1/3 of their volume.
Add a pinch of meat tenderizer to each of these tubes. Mix gently to avoid damaging the DNA.
Tilt the test tube, and pour alcohol (75% isopropyl alcohol or 95% ethanol) down the sides of the tube. Add alcohol to about 2/3 volume of the tube. You will obtain two layers. The layer at the top contains the DNA.

Now that you are done with the DNA extraction process, answer the following questions:

What purpose does blending the pea mixture serve?
Why do you need to add liquid detergent?
What role does the meat tenderizer play?
Why is alcohol added and why does the DNA get extracted in the alcohol layer?

Answers

Answer 1

Answer:

Blending is done to break open, or lyse, the cells.

DNA resides in the nucleus, which is enclosed by the nuclear membrane. The nucleus is further enclosed by the cell membrane. So, to get the DNA, we need to break open both the membranes. Cell membranes are made up of lipids and proteins. The detergent molecules to attach to the cell and nuclear membranes, causing them to break open.

Meat tenderizer contains the enzyme protease that helps cleave proteins. Even after breaking open the nucleus and cell membrane, the DNA is still protected by cell proteins. Meat tenderizer cleaves the proteins, leaving behind only the DNA molecule.

DNA is insoluble in alcohol and hence, when alcohol is added, it precipitates out the DNA.

Explanation:

PLATO Answer


Related Questions

How do you identify an element?

Answers

Answer:

element is the smallest particles which cannot be broken down.

PLEASE HELP!!!!! NEED HELP ASAP!!!!!

Write the chemical formula for the covalent compounds below
EXAMPLE- dinitrogen trioxide= N2O3

nitrogen monoxide =
dihydrogen oxide =
diphosphorus pentoxide =
sulfur trioxide =

Answers

Answer:

down below

Explanation:

NO

H20

P4O10

SO3

1. NO

2. H2O

3. P4O10

4. SO3

i have the most potential energy if i am​

Answers

Answer:

travel the world in one day

If nitrogen and hydrogen combine in a combustion reaction, what would the product of the reaction be?

CH4 + 2O2 → ____2 + 2H2O

Answers

Co2

Explanation:

CH4 (g) + 2 O2 (g) → CO2 (g) + 2 H2O (l)

Answer:

C - CO

Explanation:

Correct on EDGE

We apply the same amount of energy to 10.0-g samples of aluminum, iron, and silver which begin at the same temperature. Rank the metals from highest to lowest temperature after the heat is applied

Answers

Answer:

Ag > Fe > Al

Explanation:

The values of the specific heat capacity of the elements are given as;

Aluminum = 0.897 J/g*K  

Iron=0.449 J/g*K  

Silver=0.235 J/g*K

Specific heat capacity basically refers to how much heat is required to change the temperature of a unit mass of a substance by a degree. Elements with high specific heat capacity would require higher energy compared to elements with lower values,

Aluminium (Al) has the highest specific heat capacity, hence it wold require more energy to cause a change in temperature. Aluminium would be the lowest.

Silver (Ag) has the lowest specific heat capacity hence it would require less energy to cause a change in temperature. Silver wold be the highest.

The middle would be iron (Fe).

The rank is given as;

Ag > Fe > Al

CAN SOMEONE PLEASE HELP ME WITH THIS PLZ

Answers

Answer:

the pressure increases

A laser used in eye surgery to fuse detached retinas produces radiation with a wavelength of 6.4 x 10^-7 m. What is the frequency of the laser?
Speed of light = 3.00 x 10^8 m/s

(Hint: Remember to round your answer to the lowest number of significant figures.)


(Speed of light) / (Wavelength) = Frequency

show steps please.​

Answers

Answer:

ν ≈ 4.7 × 10¹⁴ Hz

General Formulas and Concepts:

Chem

Speed of Light = Wavelength times Frequency

c = λν c = 3.0 × 10⁸ m/s

Explanation:

Step 1: Define

λ = 6.4 × 10⁻⁷ m

Step 2: Solve for ν

3.0 × 10⁸ = 6.4 × 10⁻⁷ · ν

ν = 4.6875 × 10¹⁴ Hz

Step 3: Check

We are given 2 sig figs. Follow sig fig rules.

4.6875 × 10¹⁴ Hz ≈ 4.7 × 10¹⁴ Hz

CAN U GUYS HELP ME ON THIS ONE PLEAASE IM TOO DUMB YO ANSWER THIS ONE IM NOT EVEN LYING TOO PLUS THIS ASSIGNMENT WAS DUE LAST FRIDAY :(

CH3Br Draw out the work for all 4 steps for the Lewis structure.

Grading Criteria:
a. Correctly count the total valence electrons (2pt)
b. Correctly identify and draw the location of the central atom and surrounding atoms. (2pt)
c. Correctly draw the total amount and location of the electrons on the lewis structure. (2pt) ​

Answers

Answer:

For CH3Br, there are a total of 14 valence electrons. Hydrogen always goes on the outside, and since Carbon is less electronegative than Bromine, we'll put the Carbon in the center and the Bromine on top. We have 14 valence electrons for the CH3Br Lewis structure. Put two between atoms to form chemical bonds.

Explanation:

help !!!!!!! cell membrane plants information due soon

Answers

The cell membrane acts as a semi-permeable barrier in plants. In other words, is in charge or control of what comes in and out of the cell.

Teresa creates an experiment Washington states to read colored substances together in a container in observes the solution slowly changing from red to blue for the next eight minutes. The solution becomes completely blue after 8 minutes. What does this represent about the type of change happening in the container

Answers

The question is incomplete, the complete question is;

Theresa creates an experiment where she mixes two red-colored substances together in a container and observes the solution slowly changing from red to blue for the next eight minutes. The solution becomes completely blue after eight minutes. What does this represent about the type of change happening in the container? A chemical change started immediately and finished at eight minutes. A non-chemical change started immediately and finished at eight minutes. A chemical change occurred at four minutes. A non-chemical change occurred at four minutes.

Answer:

A chemical change started immediately and finished at eight minutes.

Explanation:

A chemical change is one in which a new substance is formed. One of the indicators that a chemical change has occurred is the change in the color of the system from red to blue.

The question clearly stated that the change occurred gradually. This means that the reaction must have started immediately the substances were mixed and went on to conclusion after eight minutes when the complete blue color appears.

what is the chem formula for chromium (V) chloride?

Answers

CrCl4
...............

You are at a carnival fun house standing 3m in front of a lens with a focal length of 2.5m. What would happen to your image if the lens was concave? What would the image look like if the lens was convex?

Answers

The convex lens would produce a virtual

Describe what happens to kinetic energy of the molecules when temperature increases.

Answers

Answer:

When the temperature of an object increases, the average kinetic energy of its particles increases. When the average kinetic energy of its particles increases, the object's thermal energy increases. Therefore, the thermal energy of an object increases as its temperature increases.

Explanation:

That is all I know.

The table below contains characteristics of four different elements. Characteristics of Four Elements Element Number of Protons Number of Valence Electrons 1 ca 4 2. 11 1 1 3 18 8 4 37 1 1 What conclusion can be made from the information above? Element 3 is a metal and Element 1 is a metalloid. Element 2 is the same element as Element 3. Element 1 has very similar chemical properties as Element 4. Element 2 has very similar chemical properties as Element 4.​

Answers

Answer:

The correct option is "Element 2 has very similar chemical properties as Element 4"

Explanation:

Both element 2 and element 4 have the same number of valence electrons; meaning they will be found in the same group (group 1) of the periodic table. Elements in the same group of the periodic table exhibit similar chemical properties. For confirmation, element 2 is sodium (based on the atomic number of 11 provided) while element 4 is rubidium (based on the atomic number of 37 provided) - the two elements are found in group 1 of the periodic table.

NOTE: Number of protons is the same as atomic number

Why do lunar and solar eclipses not happen every month?
-Eclipses only occur during full and new moons.
-Earth rotates around its axis faster than the moon.
-The moon and Earth only travel through the penumbras.
-The moon’s axis does not always align with Earth’s shadow.

Answers

Answer:

i think its the last one

Answer: The moon’s axis does not always align with the Earth’s shadow.

The moon does rotate around the Earth every month but that doesn’t mean it can get a shadow every time due to the moon’s tilted path around the Earth.

Which chemical equation below is balanced to follow the Law of Conservation of Mass?

2 H2 + Cl2 > 3 HCl
2 H2 + 2Cl2 > 2 HCl
H2 + Cl2 > 2HCl
H2 + Cl2 > HCl

Answers

Answer:

H₂ + Cl₂ → 2HCl

Explanation:

The equation that is balanced and follows the law of conservation of mass is:

 H₂ + Cl₂ → 2HCl

Based on the law of conservation of mass "in a chemical reaction, matter is neither created nor destroyed but they combine with one another".

It suggests that the number of species on both sides of the expression must be the same.

 For the given reaction:

        H₂ + Cl₂ → 2HCl

             Reactant side              Product side

H                  2                                   2

Cl                  2                                   2

Volume of water displaced

Answers

Answer:

The volume of displaced fluid is equivalent to the volume of an object fully immersed in a fluid.

What are the names, symbols, and atomic numbers of the nonmetal elements that are NOT gasses?

Answers

Seventeen elements are generally classified as nonmetals: most are gases (hydrogen, helium, nitrogen, oxygen, fluorine, neon, chlorine, argon, krypton, xenon and radon);


one is a liquid (bromine); and a few are solids (carbon, phosphorus, sulfur, selenium, and iodine). Metalloids such as boron, silicon, and germanium are sometimes counted as nonmetals.

ANSWER:

Liquid:
Bromine - Br - 35

Solid:
Carbon - C - 6
Phosphorus - P - 15
Sulfur - S - 16
Selenium - Se - 34
Iodine - I - 53


check wether all the answers are correct or not. please?​

Answers

Answer:

I dont now but I think it is correct

You spelled whether wrong

395,000 divide by 9000

Answers

Answer:

it is 395/9 =43.8888889

The lowest allowable energy state of an atom is called its .

Answers

The lowest allowable energy state of an atom is called its ground state . Hope this helps (:

whats the mass of neon

Answers

Answer:

20.17 (or if you want to round .18)

Explanation:

If you ever need help with this again the atomic mass is the number underneath the element name

Match each form of energy with its correct description.
mechanical
involving the motion of atoms
chemical
involving electrical charges
thermal
involving the position and motion of
an object
electrical
involving energy stored in chemical
bonds

Answers

Answer

involving electrical charges: electrical

involving energy stored in chemical

bonds: thermal

involving the position and motion of

an object: mechanical

involving the motion of atoms: chemical

Explanation:

almost 100 this is right, sorry if this is wrong :)

Mechanical: involved the motion of atoms
Chemical: unblocked energy stored in chemical bonds
Thermal: involves the position and motion of an object
Electrical: involving electrical charges

Need help please with this, I’ll give brainliest!!!

Answers

Answer:

38 g?

Explanation:

I'm not entirely sure, but perhaps it is 49 grams x 78.25%? This gives you an answer of 38 g using significant figures.

The element with the smallest iconic radius in the alkaline earth metals is

Answers

Answer:

Metal.

Explanation:

Metal has the smallest iconic radius in the alkaline earth metal.

A sealed can of soda contain a pressure of 1.15 atm at 23°C. What is the new pressure at 79 °C? Report your answer to the hundredths place (two decimal places) You do NOT need units in your answer

Answers

Answer:

P₂ = 1.37

Explanation:

Given data:

Initial pressure = 1.15 atm

Initial temperature = 23°C (23+273= 296 K)

Final pressure = ?

Final temperature = 79°C (79+273=352 K)

Solution:

According to Gay-Lussac Law,

The pressure of given amount of a gas is directly proportional to its temperature at constant volume and number of moles.

Mathematical relationship:

P₁/T₁ = P₂/T₂

Now we will put the values in formula:

1.15 atm / 296 K = P₂/352 K

P₂ = 1.15 atm × 352 K / 296 K

P₂ = 404.8 atm. K /296 K

P₂ = 1.37 atm

What does an oxygen atom do when becoming an ion?

Answers

It gains 2 electrons from another atom so it becomes an anion.

How can the gravitational potential energy of an object be changed?

Answers

Answer:

Gravitational potential energy may be converted to other forms of energy, such as kinetic energy. If we release the mass, gravitational force will do an amount of work equal to mgh on it, thereby increasing its kinetic energy by that same amount (by the work-energy theorem).

Explanation:

if one species of rabbit, the gene for brown fur ( B) is dominant, while the gene for black fur ( b) is recessive. One rabbit has the dominant allele for brown fur and the recessive allele for black fur. Another rabbit has two recessive alleles. If the two rabbits have offspring, what are the odds of one of the offspring having brown fur?

Answers

Answer:

1/2

Explanation:

According to this question, the gene coding for fur color in rabbits contains two alleles viz: alelle for brown fur ( B) is dominant, while the allele for black fur ( b) is recessive. This means that an heterozygous rabbit (Bb) will have brown fur.

Based on the question, one rabbit has the dominant allele for brown fur and the recessive allele for black fur i.e genotype Bb, another rabbit has two recessive alleles i.e. genotype bb. If these two rabbits cross i.e. Bb × bb, the proportion of the offsprings produces will have Bb, Bb, bb and bb genotype.

Bb is dominant, hence, 1/2 of the offsprings will be brown furred.

one species of rabbit, . the two rabbits have offspring, what are the

Electromagnetic waves will travel the SAME SPEED (speed of light) WITHIN a medium as it does WITHOUT a medium. Question options: True False

Answers

Answer:

True

Explanation:

The speed that electromagnetic waves will travel within any medium is the same as those without a medium. The speed of electromagnetic wave is a constant and does not depend on the material of the medium.

Electromagnetic waves are waves that do not require a material medium for their propagation. They are able to pass through vacuums and mediums with particles at constant speed. It is mechanical waves whose speed is medium dependent. Mechanical waves have varying speed based on the mediums they move through.
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