A 1.0-kb DNA fragment from the 5' end of the mouse gene is examined by DNA footprint protection analysis. Two samples are end-labeled with 32P, and one of the two is mixed with TFIIB, TFIID, and RNA polymerase II. Both samples are exposed to DNase I. The results are displayed below. The DNA exposed to proteins is run in the right-hand lane of the gel. Control DNA is in the left-hand lane.
Required:
What length of DNA is bound by the transcriptional proteins?
Answers
Answer:
Explanation:
Proteins, such as RNA (ribonucleic acid) polymerase, were loaded into lane 2. TFIIB and TFIID are two forms of TFII. These proteins attach to the fragment of DNA. Furthermore, these proteins shield DNA from the DNase enzyme, which may trigger DNA to split. As a result of the above findings, we may conclude that the inserting proteins(RNA polymerase, TFIID and TR 8 proteins) cover the area between 500 and 800 bp (base pair) in the DNase I footprinting experiment.
As a consequence, the length duration is 300bp since the promoter area is about 500-800bp and is not digested by DNase after incubation of inserting proteins.
Related Questions
Explain why atmospheric concentrations of carbon dioxide are expected to increase in the future.
Answers
Answer:
because of factories and cars and smoking
Explanation:
Water flow improves in the aftermath of a forest fire because
rain always puts out fires.
no living organisms survive fires, decreasing demand.
they clear the land, allowing more water to flow over it.
much of the water in a local watershed will be used to fight the fire.
Answers
A major impact of combustion on water quality is the potential for increased precipitation. Runoff can carry suspended soil particles, dissolved inorganic nutrients, and other materials into nearby rivers and lakes, degrading water quality and damaging fish habitat.
Park Williams, an associate professor of geography at UCLA and lead author of the study, said wildfires burn vegetation that would otherwise draw water from the ground and block precipitation before it reaches the ground. said to improve the flow.
Wildfires increase the vulnerability of watersheds to flooding and erosion, causing short-term and long-term damage to water supplies. may have an impact. The increased cost of treatment, the need for alternative medicine, and reduced storage capacity. Fire as a destructive force can rapidly consume large amounts of biomass and cause adverse effects such as soil erosion, post-fire water runoff, and air pollution.
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Answer: The correct answer is they clear the land, allowing more water to flow over it.
Explanation: Confirmed correct.
Forest fires clear the underbrush and branches from the forest floor, improving the water flow and saturation.
Anorexics are often hungry but refuse to eat, which is misleading because the literal meaning of anorexia is “__________.” A. unhealthy dieting B. excessive hunger C. excessive weight loss D. loss of appetite
Answers
Answer: D
Explanation:
Answer:
D edge 2021
Explanation:
Extensive damage to oligodendrocytes in the CNS could result in reduced speed of nerve impulses. a breakdown of the blood-brain barrier. loss of the structural framework of the brain. inability to produce scar tissue at the site of an injury. decreased production of cerebrospinal fluid.
Answers
Answer:
Loss of the structural framework of the brain.
Explanation:
Damage to oligodendrocytes in the central nervous system leads to the loss of the structural framework of the brain. oligodendrocytes produce the insulating sheath of axons and we know that axons are the nerve fiber that conducts electrical impulses away from the neuron's cell body. Axons are the primary transmission lines of the nervous system that carries impulse throughout the body so if the oligodendrocytes are damaged then our brain did not work properly.
If oligodendrocytes are damaged, the myelin sheaths around axons can be destroyed. This can lead to a loss of the structural framework of the brain, as the axons will no longer be properly insulated. Hence option C is correct.
Oligodendrocytes are glial cells in the central nervous system (CNS) that are responsible for myelinating axons. Myelin is a fatty sheath that surrounds axons and helps to speed up the conduction of nerve impulses.
The loss of the structural framework of the brain can cause a variety of neurological problems, including:
Reduced speed of nerve impulses. As mentioned above, myelin sheaths help to speed up the conduction of nerve impulses. Without myelin sheaths, nerve impulses will travel more slowly, which can cause problems with coordination, movement, and sensation.
Increased risk of seizures. Seizures are caused by abnormal electrical activity in the brain. When the myelin sheaths around axons are damaged, it can increase the risk of seizures.
Cognitive impairment refers to a decline in thinking and memory skills. Damage to oligodendrocytes can lead to cognitive impairment, as the axons will no longer be able to function properly.
Therefore, option C) loss of the structural framework of the brain is correct.
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Help asap plsss no link or websites I think brainley has a bot problem
An airplane flies with a constant speed of 760 km/h. How far can it travel in 2 1/2 hours? Distance =
Answers
Answer:
I would say 1520 miles is your answer
What adaptation helps the pine tree reduce water loss?
leaves
thorns
Seeds in cones
Woody stems
Answers
Answer:hojas
Explanation:
Answer:
A
Explanation:
Pine trees thankfully have needles, which are thin, waxy leaves that enable them to make food year-round. The waxiness of the needles helps evergreens not lose water to evaporation.
B. What is the mass number and charge of the emitted positron?
Mass number:
Charge:
Answers
Answer: A positron or antielectron is the antimatter counterpart to an electron. A positron has the equal or same mass as an electron and a spin of 1/2, but it has an electrical charge of +1.
Explanation:
help me plz
Living organisms are classified in different ways. Cells can help determine an organism's classification. Describe how an organism with plant cells might be classified differently than an organism with animal cells.
Answers
Explanation:
A plant cell consists of one large vacuole that maintains the shape of the cell and stores nutrients. Animal cells, on the other hand, have multiple smaller vacuoles. Both plant and animal cells have a cell membrane, but only the former has a cell wall.
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After an initial infection of the measles virus, B-cells recognize the measles virus in your body. How is this helpful in human immune response? (SC.912. L.14.52)
ws
O The B-Cells transfer this recognition to T-cells, which will then devour the viruses
o The B-cells use this recognition to defend the body against the B-cells more quickly recognize and respond to any other virus that invades the body
O The B-Cells more quickly recognize the response to any other virus that invades the body
O The B-cells produce antibodies more quickly if the measles virus is encountered again
Answers
Answer: it’s D
Explanation:
How does the atmosphere help to make Earth livable? Check all that apply.
causes the seasons
contains oxygen
provides warmth
creates important nutrients
blocks harmful energy from the Sun
Answers
Answer:
- contains oxygen
- provides warmth
- blocks harmful energy from the Sun
Explanation:
this is the answer hope this helps (^_^)
Answer:
the one above me is right
Explanation:
A solid is that form of matter that possesses the following
characteristics___.
a Particles vibrate around fixed axes
b Definite shape
C Definite volume
d All of the above
Answers
what is a scienctific theory
Answers
Answer:
" A scientific theory is a well-substantiated explanation of some aspect of the natural world, based on a body of facts that have been repeatedly confirmed through observation and experiment. Such fact-supported theories are not "guesses" but reliable accounts of the real world."
Explanation:
- Eijiro <3
Every cell in your body was produced through successive rounds of mitosis, starting from the zygote.
a. True
b. False
Answers
Answer:
I think that the correct answer is True
Why would a rough, steep mountain habitat have a higher rate of speciation
than a large, open grasslands habitat?
A. The mountain has less variety in the environment.
B. The mountain would cause less fragmentation.
C. The mountain has wider range of environments.
D. The mountain has more resources.
Answers
Answer:
C. The mountain has wider range of environments.
Explanation:
Steep mountain habitat have a higher rate of speciation than a large, open grasslands habitat The mountain has wider range of environments.
What is Grassland habitat?There are various locations and climates on earth where grassland environments can be found. Depending on where in the world they are and the type of climate there, they may also be referred to as prairies, savannahs, steppes, or pampas.
Because there are few places for animals to hide from predators in grasslands, they have developed underground burrows or grown tall enough to look over thick grasses and shrubs.
Many grasslands have been used for farming because they are suitable for raising crops and providing food for cattle. Yet, this means that grassland animals can no longer reside there.
Therefore, Steep mountain habitat have a higher rate of speciation than a large, open grasslands habitat The mountain has wider range of environments.
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Protein phosphatase 1 (PP1) catalyzes a reaction which yields products that regulate cell division; consequently, PP1 is a possible drug target to treat certain types of cancers. The PP1 enzyme cata- lyzes the hydrolysis of a phosphate group from myelin basic protein (MBP). The reaction is shown below:
PP1 MBP-phosphate-→ MBP+P,
The activity of PP1 was measured in the presence and absence of the inhibitor phosphatidic acid (PA). The concentration of PA was 300 nM. vo without PA vo with PA released . (mg/mL) mL"1 . min1) mL-1 . min-1) 0.00381 0.00620 0.00931 0.0140 0.010 0.0209 0.0335 0.0419 0.0838 0.015 0.025 0.050
(a) Use the data provided to construct a Lineweaver-Burk plot for the PP1 enzyme in the presence and absence of PA. What kind of inhibitor is PA?
(b) Report the KM and Vmax values for PP1 in the presence and absence of the inhibitor.
Answers
Answer:
Explanation:8iikk
Circle the trisomy shown on
this karyotope.
Answers
Answer: 13 is a trisomy
Explanation:
A solid is that form of matter that possesses the following
characteristics
a
Particles vibrate around fixed axes
b Definite shape
C Definite volume
d All of the above
Answers
This model of the cell cycle includes two arrows that each represent a process in the cycle. What do the two arrows represent?
A.Arrow 1 represents interphase, and Arrow 2 represents mitosis.
B.Arrow 1 represents meiosis, and Arrow 2 represents prophase.
C.Arrow 1 represents prophase, and Arrow 2 represents interphase.
D.Arrow 1 represents mitosis, and Arrow 2 represents meiosis.
Answers
About the question:
You will find the model of the cell cycle in the attached files
Answer:
A. Arrow 1 represents interphase, and Arrow 2 represents mitosis.
Explanation:
When we talk about the cell cycle, we refer to the interphase and the cell division (mitosis or meiosis).
Interphase: Represented by arrow 1.
The interphase is the previous step before mitosis occurs. The interphase is conformed of the G1, S, and G2 stages.
• During the G1 stage, it occurs a high intense biochemical activity. The cell duplicates its size, and the organelles and other molecules and cytoplasmatic structures also double. Some structures, such as microtubules and actin filaments, are synthesized from zero. The endoplasmic reticulum increases in size and produces a membrane for the Golgi complex and vacuoles, lysosomes, and vesicles. During this stage, the pair of centrioles separate, and each centriole duplicates. Mitochondria and chloroplasts also replicate.
• During the S stage, the DNI replication process occurs. This is the only stage in which the DNI molecule replicates. The synthesis of histones and other associated proteins also occurs.
• G2 stage is the interphase last step. DNI molecule begins to slowly condensate. The centrioles duplication process completes. The spindle fibers get assembled.
Once the whole interphase is completed, mitosis occurs.
Mitosis: Represented in the cycle by arrow 2.
Mitosis is a process by which, from a diploid somatic cell (2n), two daughter diploid cells (2n) are produced. During mitosis, the whole-cell first duplicates and then separates. Mitosis is divided into four phases (these phases are shown in the cycle with different gray color tones). During prophase, chromosomes condensate, and the nuclear membrane breaks. During the metaphase, fibers of the spindle apparatus take chromosomes toward the equatorial cell plane, where they line up. Sister chromatids are holden together until they reach the Anaphase, during which specialized enzymes break the bonds between chromatids and separate them. Each chromatid migrates to one of the poles. In telophase, the new chromosomes are already in the corresponding poles, and the nuclear membrane forms again in each pole. Finally, cytokinesis occurs.
Is a forest area that has been washed away by a flood a primary or secondary area of succession?
Answers
Answer:
I'm pretty sure it's secondary
Explanation:
Secondary succession is a major disturbance such as a fire or a flood
The chemical properties of an element
are determined by its
A- atomic mass
B-proton number
C-electron arrangement
D- atomic size
Answers
Answer:
C. electron arrangment
Explanation:
Electrons are the subatomic particles that influence an element's reactivity since they are exchanged during reactions.
Question: 1-7
Which of the following is NOT a function of the stem in a plant?
o absorb water
store nutrients
produce new plant cells
O support flowers and leaves
Answers
Answer: Absorb water
Explanation: i took the quiz the other guy is wrong
Absorbing the water from soil is function of root and not a stem. Thus, option B is correct.
What is stem?The structural part of plant, that supports leaves, flowers, and fruits is called as stem. Stems are frequently differentiated for storage, asexual reproduction, protection, or photosynthesis.
The stem is divided into nodes and internodes. Node consist of buds and leaves, that can grow into the branches. It also gives rise to adventitious roots. The function of internode is to distance one node from another.
Stem supports and uprights leaves, flowers and fruits. The stem keeps the leaves in the light and provides the plant with a place to hold the flowers and fruit. It transports fluid between xylem and phloem roots and shoots. Therefore, transportation of water and nutrients is the function of stem, and not absorption. Hence, option B is correct.
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Describe the force of Earth’s gravity.
Answers
Answer
Gravity, also called gravitation, in mechanics, the universal force of attraction acting between all matter. ... On Earth all bodies have a weight, or downward force of gravity, proportional to their mass, which Earth's mass exerts on them. Gravity is measured by the acceleration that it gives to freely falling objects.
Describe passive and active freezing protections that could be set in place for an orchard of apples being grown in New York State and explain why they might be effective.
3 SENTENCES AT THE LEAST
Answers
Answer:
A good answer could include planting the trees on the north-facing flank of a hill (passive) and providing overhead irrigation to allow the grower to give plants an ice coating (active). Apples grown in New York face their strongest threat during bloom-time. North-facing plantings partway up a hillside delays bloom-time to later in spring, reducing the odds of the plants experiencing a hard frost. Irrigation allows the grower to take advantage of the severe overall cold that can be associated with a freeze in upstate New York by using the cold to create a protective ice layer that can preserve the blossoms.
Explanation:
this is the example answer. do what you will w it
The passive and active freezing protections can be done by planting facing north hill and providing overhead irrigation respectively.
What is Protection?This is the process of guarding something against damage from an external force.
The passive and active freezing protections are effective as it minimizes the risk of freezes and frosts during spring blooms.
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Friction reduces efficiency because energy is lost as ____.
a.
work
c.
gravity
b.
heat
d.
thrust
Please select the best answer from the choices provided
A
B
C
D
Answers
Answer:
option (b) heat is right answer
Hydrogen bonds are formed as a result of which of the following? A. Ionic bonds b. Nonpolar covalent bonds c. Polar covalent bonds d. None of these are correct
Answers
Answer:
C
Explanation:
I believe they're opposite.
One thousand offspring were typed. This cross produced 430 red-eye, wild-type wing flies, 65 purple-eye wild-type wing flies, 60 red-eye, vestigial wing flies, and 445 purple-eye, vestigial wing flies. What is the distance between the loci for purple eyes and vestigial wings in centiMorgans (map units)
Answers
Complete question:
In Drosophila, red eyes is wild-type and purple eyes are mutant. Full-size wings are wild-type and vestigial wings are mutant. Flies with wild-type pr pr vg vg genotypes were crossed with double mutant flies with a pr-pr- vg-vg- genotypes. The F1 flies had pr pr- vg vg- genotypes. The F1 flies with heterozygous genotypes were crossed with double mutant flies with a pr-pr- vg-vg- genotypes One thousand offspring were typed. This cross produced 430 red-eye, wild-type wing flies, 65 purple-eye wild-type wing flies, 60 red-eye, vestigial wing flies, and 445 purple-eye, vestigial wing flies. What is the distance between the loci for purple eyes and vestigial wings in centiMorgans (map units)
Answer:
Distance between genes → 12.5 MU = 12.5 cM.
Explanation:
You will find the complete explanation in the attached files due to technical problems.
A kitten embryo is growing inside the uterus of a cat. What is happening to the cells of the kitten embryo?
A) Mitosis, the cells are differentiating
B) Meiosis, the cells are differentiating
C) Meiosis, the genes are being shuffled around
D) Mitosis, genes are being shuffled around
Please answer and don't put fake answer I really need this right.
Answers
Answer:
A
Explanation:
the cells will start to split causing growth in the embryo
Help me please!!!!!!!!!!!!!!!!!
Answers
Explanation:
B one is the correct answer
Answer:
I think it's X
Explanation:
what is type of cell division by which sperms are produced
Answers
Answer:
Meiosis man
Explanation:
Just meiosis
Answer:
Meiosis is the type of cell division that creates egg and sperm cells.
Explanation:
How does stimulus become a memory? stay safe god bless.
Answers
Memories will occur when specific groups of neurons have been reactivated. The stimulus results in any brain in a particular pattern of neuronal activity. Some neurons start to become active in either more or less a particular sequence. Retrieval Cue; a stimulus for remembering information.