which technology has been commonly utilized by scientists to manipulate heritable information by producing large amounts of identical recombinant dna molecules? gel electrophoresis gene cloning artificial selection restrictive enzyme analysis

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Answer 1

The technology that has been commonly utilized by scientists to manipulate heritable information by producing large amounts of identical recombinant DNA molecules is gene cloning.

Gene cloning is the process by which we insert a section of DNA into a cell and then let the cell undergo mitosis, creating copies of that genetic information. Gene cloning is different from organism cloning, which uses an organism's DNA to produce a genetically identical organism because it only produces cells, not an entire organism.

Cloning of any DNA fragment involves four steps in the traditional restriction enzyme digestion and ligation cloning protocols: isolation of the desired DNA (or target DNA ), ligation, transfection, or transformation.

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Related Questions

do nutrients and oxygen pass through the walls or your artieries and into your cells

Answers

Answer:

Yes

Explanation:

Trust me bro. Look it up on Bi(n)g not Go(o)gle

describe the use and method of iodine solution test from starch

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Using iodine to test for the presence of starch is a common experiment. A solution of iodine (I2) and potassium iodide (KI) in water has a light orange-brown color.

If it is added to a sample that contains starch, such as the bread pictured above, the color changes to a deep blue.

explain why having different enzymes for one or more steps is critical for regulation of the two pathways.

Answers

To control the entire reaction speed, the activation of these catalysing enzymes must be controlled.

Why does the body need so many different enzymes?

Due to its extensive range of intricate metabolic processes and activities, the human body requires a wide variety of enzymes. The different metabolic chemical reactions that occur are catalysed by the enzymes, and once an enzyme has catalysed a reaction, it is no longer functional and must be replaced.

By employing the byproducts of enzymatic processes to block more enzyme activity, cells have evolved to use feedback inhibition to control enzyme activity in metabolism. Anabolic and catabolic processes, for example, must continue in accordance with the needs of the cell.

Enzymes tend to be tightly regulated because they direct and govern a cell's metabolism. We'll examine elements that can influence or regulate enzyme activity in this article.

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HELP PLEASE!!! Match the correct term with the corresponding definition or description.

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The image below has the solution. To read the text, enlarge it.

Match the correct term with the corresponding definition or description.

1. Matter- anything that has mass and takes up space

2. Rock formation - a region of rock that formed together as a single rock type

3. Rock cycle - Process where any rock type can be changed into any of the other type and back again depending on conditions

4. Erosion- movement of rock pieces and or sail from one place to another by natural processes

5. Deposition - smaller pieces of rocks are spread out across area

6. Compaction- the process of sediment being buried and pressed together

7. Cementation- eventually pieces get stuck together and becomes a rock

8. Energy - the ability to make things move or change

What is Rock?

Any naturally occurring solid mass or mineral aggregate is referred to as rock. It is divided into groups based on the minerals it contains, its chemical make-up, and how it is produced.

The region of rocks that formed as a single rock type is known as the rock formation. Any rock type can be transformed into any other type of rock and back again through the rock cycle process, depending on the circumstances. Erosion is defined as the natural movement of rock fragments and/or sail from one location to another.

Thus, matching the correct term with the corresponding definition or description.

1. Matter- anything that has mass and takes up space

2. Rock formation - a region of rock that formed together as a single rock type

3. Rock cycle - Process where any rock type can be changed into any of the other type and back again depending on conditions

4. Erosion- movement of rock pieces and or sail from one place to another by natural processes

5. Deposition - smaller pieces of rocks are spread out across area

6. Compaction- the process of sediment being buried and pressed together

7. Cementation- eventually pieces get stuck together and becomes a rock

8. Energy - the ability to make things move or change

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Elisa’s diabetes causes her to have lower than normal numbers of glucose molecules in her cells. You already wrote about how this makes her tired. Now, write an explanation that answers the question how could elisa's diabetes also affect her body’s ability to grow and repair cells? use the following sentence to start your explanation, or use a sentence of your own. Diabetes could affect how well elisa's cells can grow and repair themselves. Word bank glucose oxygen cellular respiration amino acid protein energy.

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Diabetes in the disease where cell's ability to uptake glucose efficiently is affected. As a result the cell will not able to produce energy by glucose breakdown and then whole process of performing cellular respiration in the presence of oxygen will shut down. Thus is the reason why the cell will not be able to produce the necessary proteins for body's growth and repair.

Diabetes is of two types. Diabetes 1 is the insulin-dependent diabetes where the body does not produce enough insulin. Diabetes 2 is the inability of the body cells to uptake glucose efficiently.

Cellular respiration is the breakdown of glucose in the presence of oxygen to yield energy for the cells. This energy is essential to perform the various functions of the cell and make it living.

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Ddt was once considered a silver bullet that would permanently eradicate insect pests. Instead, ddt is largely useless against many insects. Which of these would have prevented this evolution of ddt resistance in insect pests?.

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Non of the insect pests would have genetic variations that resulted in DDT resistance.

Dichlorodiphenyltrichloroethane (DDT) is an insecticide used in agriculture. United States banned the use of DDT in 1972. They are responsible for causing many  Human health effects. EPA also issued an order for DDT on its adverse environmental effects, to wildlife, as well as on human health .

DDT have been broadly studied for their toxicity and carcinogenicity in animals and humans and shown to have an endocrine abolition potential affecting reproductive system although the effects may vary among animal species in correlation with exposure levels.

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which structure is highlighted? external intercostal muscle sternum internal oblique muscle ribs and costal cartilage

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Ribs and coastal cartilage is highlighted.

Costal cartilages are part of hyaline cartilage which serve to drag out the ribs forward and contribute to the elasticity of the walls of the thorax.

Costal cartilage present between your ribs and your breastbone permits  the rib cage to be more flexible and also increases the ability to expand when you breathe.

This impact your rib cage that can cause a tear in this costal cartilage. Any damage in Rib cartilage creates a very  painful situations. It often leads to severe pain while taking deep breaths, and even more pain while coughing.

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what advantage might a segmented worm have over a non-segmented worm have in terms of movement?

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The advantage of segmentation in segmented worms over non-segmented worms is strong and flexible movement.

Segmented worms are worms that have a segmented body with repeating units. Unsegmented worms are worms that do not have segmentation.

The main advantage of segmented worms over unsegmented worms is that segmentation allows these worms to move freely.

Each segment has its nerve and muscle tissue. Each has bristles called setae. When a worm moves through dirt, the bristles or setae aid in anchoring and controlling it.

While the other portion of the worm's body protrudes forward, the bristles firmly anchor one part of the worm to the ground.

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which of the following is an antigen that would be recognized by a helper t cell? a. a bacterium inside a cell b. a virus inside a cell c. an uninfected human cell d. the malaria parasite inside a red blood cell e. a bacterium outside a cell

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A bacterium inside a cell is an antigen that would be recognized by a helper t cell

What is an antigen ?

Any chemical that triggers the body to mount an immunological defence against it. Toxins, chemicals, germs, viruses, and other foreign substances are examples of antigens. Cancer cells and other body tissues and cells contain antigens that might elicit an immune response.

When class II MHC protein is present, often on the surface of an antigen-presenting cell, T helper cells can detect viral peptides (APC). If the binding affinities are high enough, these interactions lead to the activation, proliferation, and differentiation of T helper cells.

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which of the following choices list the correct order of structures through which urine passes on its way out of the body?

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The correct sequence of structures through which urine passes as it leaves the body: Kidneys → urethra → bladder→ ureters.

Urine is the by-product of metabolic processes that are processed in the kidneys and excreted from the body through the urinary tract. The process of forming urine consists of three stages, namely filtration (filtering), reabsorption, and augmentation or secretion (collection).

Excretory organ function:Kidneys are where urine is formed.The ureter is a tube that carries urine from the kidney to the bladder.The bladder is a system of urinary tracts in the form of hollow sacs whose job is to collect fluid that has been excreted by the kidneys and will be excreted as urine.The urethra is the tube that carries urine from the bladder to the outside of the body.

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Even in the absence of any mutations or crossing over, what process would still ensure genetic variability in the gametes produced by a single individual?.

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Even in the absence of any mutations or crossing over, option C: independent assortment would still ensure genetic variability in the gametes produced by a single individual.

Each gamete has a unique DNA set because of recombination and independent assortment during meiosis. As a result, the resulting zygote has a unique set of genes. The chromosomes randomly move to different poles during meiosis due to a process called independent assortment. After meiosis, a gamete will have 23 chromosomes, but independent assortment means that each gamete will have one of numerous possible chromosome combinations. Thus, even without mutations, or recombination, or crossing over, genetic variations can be produced in an individual.

This rearrangement of genes into distinctive combinations boosts genetic diversity in a population and accounts for the differences between siblings of the same parents.

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Complete question is:

Even in the absence of any mutations or crossing over, what process would still ensure genetic variability in the gametes produced by a single individual?

None of these.

Random fertilization

Independent assortment

Separation of sister chromatids.

Mitosis

which statement regarding the lymphatic system is true?
a. Lymphatic capillaries possess one-way valves. These valves permit the uptake of fluid from the body but do not allow the fluid to low back out of the capillaries into the intracellular spaces.
b. The pancreas contains lymphocyles and macrophages that monitor the blood for microbes.
c. Lymph nodes are sites of activation of neutrophils, which destroy microbes.
d. The thymus serves as the site for activation of B cells.

Answers

The lymphatic system aids in the uptake of interstitial fluid and activation of lymphocytes.

The lymphatic system is a network of thin tubes and lymph nodes located throughout the body. These tubes are known as lymphatic vessels or lymph vessels. The lymph system is an essential component of our immune system. It aids in the fight against bacteria and other infections.

Tissues and organs that generate, store, and transport white blood cells that fight infections and other diseases. The bone marrow, spleen, thymus, lymph nodes, and lymphatic vessels are all part of this system. Lymph fluid flows through lymph vessels and into the bloodstream.

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where do we find high nutrient availability for photosynthesis? the tropics with a strong thermocline, iron rich sediments of the bathyal zones, surface zones of weaker thermoclines/stratification, near the surface at the centers of gyres

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The places where we will get high nutrient availability for photosynthesis are the surface zones of weaker thermoclines. So, the third option is the best option.  

Gyres are remote areas that are not suitable for photosynthesis at all. Phytoplanktons that fix most of the Earth's oxygen through photosynthesis are scarce in such regions as well.  Bathyal zones are also not good for photosynthesis since no light really reaches these spots. They are really deep and dark, which means no sunlight for plants.

Areas with a strong thermocline are no good because they prevent the mixing of nutrients in the water. This means that regions of weak thermocline are what suit nutrient availability the best.

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Using hierarchical levels,explain how it is possible to create different types of cells and organisms

Answers

cells make up tissue, tissue make up organs, organs make up organ systems, and organ systems make up organisms.

how do both jetties and seawalls help manage beach erosion?

Answers

Jetties and seawalls help manage beach erosion by acting as physical barriers. These physical barriers absorb much of the force which is carried by the ocean's water as it flows towards the land. This reduces the water's capacity to erode the shoreline. Jetties and seawalls are man made structures that lie beyond the land's edge.

______ synthesis begins at a site within a chromosome called the origin of replication.

Answers

DNA The origin of replication is a location on a chromosome where synthesis starts.

What do you mean by DNA replication?

It is crucial that each daughter cell receives an exact copy of the DNA when a cell divides. DNA replication is the process that makes this happen. Before the cell reaches mitosis or meiosis, during the synthesis phase, also known as the S phase, of the cell cycle, DNA replication takes place.

An indication of how DNA gets duplicated was given by the clarification of the double helix's structure. Recall that cytosine pairs with guanine while adenine pairs with thymine. The two strands are so complimentary to one another. For instance, a complimentary DNA strand will have the nucleotide sequence TCAGTACT for a strand of DNA having the nucleotide sequence AGTCATA.

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Which of the following represents a virus species name?

a. herpes simplex virus
b. herpesviridae
c. picornavirus
d. enterovirus
e. lentivirus

Answers

The correct choice is (B) Herpesviridae virus. Herpesviridae is a big or we can say large family of some DNA viruses that may cause infections and may have certain diseases in animals, as well as including humans.

Prevention: A vaccine was recently approved in the United States to prevent infection with the varicella-zoster virus. Vaccines against herpes simplex virus 2 and cytomegalovirus have been extensively evaluated in field trials. Passive immunization with immunoglobulin or hyperimmune globulin is used to prevent infection or as an adjunct to antiviral therapy.

Process: Infections with herpes simplex viruses 1 and 2 and varicella-zoster virus are currently the most treatable. Acyclovir, valacyclovir, and famciclovir are all approved treatments. Ganciclovir is used to treat cytomegalovirus retinitis. The B virus appears to respond to one of these drugs. There is still no cure for Epstein-Barr virus or human herpesvirus 6, 7, or 8 infections.

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True or False) DNA does not have to break apart to be copied.

Answers

Answer:

false

Explanation:

1. if you used information from two different data sources, the number of cases of bacterial infections were obtained from the local health department, while the data regarding the number and types of fish products sold at local stores were obtained from the epiland fishing and wild game office. what type of epidemiological study would it be if you used two different types of information to test whether the consumption of fish products from epiland lake are associated with a higher risk of bacterial infections? 2. list two potential epidemiologic study types that would be suitable if all of the data came from the same source (i.e. one study) and it was used to test whether the consumption of fish products from the epiland lake are associated with a higher risk of bacterial infections?

Answers

Gram staining and observational studies need to be done in order to get the idea of the particular epidemiology.

Bacterial infections have a large impact on public health. Disease can occur at anybody site and can be caused by the organism itself or by the body's response to its presence. Bacteria are transmitted to humans through air, water, food, or living vectors

   In order to know more about the bacteria and its pathogenicity, we perform gram staining. Pathogenicity is the ability of a bacteria or virus or any microorganism to cause the disease in an individual.

    Gram staining of a bacteria gives the idea of how deadly the bacteria or the virus is. The principal modes of transmission of bacterial infection are contact, airborne, droplet, vectors, and vehicular. Preventive measures have a dramatic impact on morbidity and mortality. Such measures include water treatment, immunization of animals and humans, personal hygiene measures, and safer sex practices. Bacterial resistance to antibiotics is a growing concern mandating their prudent use.

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a researcher has sectioned a mouse embryo and notices that certain muscle components (especially myosin) aggregate around the early-stage cartilage of the ribs. which of the statements is true of these presumptive muscles? please choose the correct answer from the following choices, and then select the submit answer button. answer choices because they associate with the ribs, these muscles are likely components of the diaphragm. because they associate with the ribs, these muscles are likely developing intercostal muscles. these muscles likely only serve a supportive role for the ribs and will play no role in breathing. these muscles (when they are fully developed) will help to maintain a constant volume in the thoracic cavity.

Answers

b. Since they associate with the ribs, these muscles are likely developing intercostal muscles.

Intercostal muscles is groups of muscles that found in between the ribs, and helps to move chest wall. Intercostal muscles primary function is helping in mechanical aspect of breathing by expanding and shrinking the  chest cavity.

Collagen is a protein that makes skin cells strong and resilient. They  provides structural support to the extracellular space of connective tissues. Because of its rigidity and resistance to stretching, it is the perfect combination for skin, tendons, bones, and ligaments.

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The introduction of the automobile in the United States encouraged the creation of what American cultural landscape?

Answers

Answer: thousands of miles of highways and roads.

Explanation:

precentage of each type of base

Answers

Answer:

Human DNA is 30.9% A and 29.4% T, 19.9% G and 19.8% C.

Explanation:

The rule constitutes the basis of base pairs in the DNA double helix: A always pairs with T, and G always pairs with C

For which of the following conditions would you MOST likely encounter a ventricular peritoneum shunt?



Epidural hematoma

Hydrocephalus

Cerebral palsy

Subdural hematoma

Answers

You would most likely come across a ventricular peritoneum shunt if you had hydrocephalus under the conditions listed below.

An explanation of ventricular pleural shunts

Introduction: Ventriculo-pleural reroutes (VPLS) drain extra cerebrospinal fluid to the pleural cavity as a different method of treating hydrocephalus. Pleural effusion will occur and accumulate as a result of imbalances between CSF production and absorption.

A ventriculoperitoneal shunt lasts how long?

After several years, especially among younger children, VP shunts are likely to need to be replaced. The shunt in a newborn has a two-year lifetime on average. A shunt replacement may not be necessary for adults or kids older than 2 for 8 or more years.

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suppose these two chromosomes are found in a cell nucleus during prophase. what is relationship between chromatids

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Suppose those chromosomes are discovered in a cell nucleus throughout prophase. A is equal to B, and C is identical to D in dating among chromatids.

During prophase, the complicated DNA and proteins contained within the nucleus, called chromatin, condenses. The chromatin coils and turns increasingly more compact, ensuing in the formation of visible chromosomes.

In the course of prophase II, the chromosomes condense, and a brand new set of spindle fibers bureaucracy. The chromosomes begin transferring closer to the equator of the cellular. at some stage in metaphase II, the centromeres of the paired chromatids align alongside the equatorial plate in each cell.

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how does dna code for an organism?
PLS ANSWER

Answers

Explanation:

Genetic Code

Each gene's code uses the four nucleotide bases of DNA: adenine (A), cytosine (C), guanine (G) and thymine (T)

you were provided with two different ssdna oligonucleotides from which you produced dsdna. what procedure did you perform that facilitated the assembly of dsdna? what properties of dna made this possible?

Answers

Procedure performed that facilitated the assembly of dsDNA is : Golden Gate Assembly (GGA).

What is Golden Gate Assembly?

Golden Gate assembly is also known as Golden Gate cloning and is created by Carola Engler and colleagues in 2008.

Golden Gate Assembly is an efficient way to quickly assemble multiple DNA sequences or parts into a single plasmid.

Golden Gate assembly is a molecular cloning method that allows a researcher to simultaneously assemble multiple DNA fragments into a single piece using Type IIS restriction enzymes and T4 DNA ligase.

Golden Gate assembly utilizes a Type IIS restriction enzyme which cleaves outside of  non-palindromic recognition sequence and T4 DNA Ligase in a  single-tube reaction.

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__ refers to the fact, that, if the conditioned and unconditioned stimuli are not paired for a given number of trials an organism will stop exhibiting the conditioned response.

Answers

Extinction refers to the fact that, if the CS and UCS are not paired for a given number of trials, an organism will stop exhibiting the CR.

When the entire species dies or gets eradicated it is called extinction. When the number of species decreased due to environmental, as well as when individual species members undergo evolutionary changes (genetic inbreeding, poor reproduction, decline in population numbers) extinction occurs.

Extinction rates vary greatly.

The majority of species that have ever lived on Earth have gone extinct, but the fossil record reveals five particularly large extinctions, known as mass extinction events, many species extinct due to this. The majority of extinctions, which happen continuously and are referred to as background extinctions, are distinguished from these glaring declines in diversity by the term "mass extinction."

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If a cell is no longer able to differentiate into any type of tissue, it has become ________.

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If a cell is not able to differentiate into any type of tissue it has differentiated.

Generic embryonic cells become specialized cells through cell differentiation. Gene expression is a process that causes something to happen. The precise combination of genes that are turned on or off (expressed or repressed) determines how a cell functions, and this is known as gene expression. Certain signals in your body, both inside and outside of your cells, cause gene expression.

All multicellular creatures' development, growth, reproduction, and long-term viability depend on the regulation of cell differentiation. Not only neurons but other cells also lose their capacity to divide as they age.

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a higher level of hemoglobin in the blood increases the blood's density. this is the basis for a simple test that can be used to see if a prospective blood donor has a high enough hemoglobin level to donate safely. a drop of blood is placed in a copper sulfate solution, and the time for the drop to sink to the bottom is measured. if this time is too long, the hemoglobin level is too low. part a explain how this test works. match the words in the left column to the appropriate blanks in the sentences on the right. make certain each sentence is complete before submitting your answer. resethelp

Answers

The density of blood rises as hemoglobin concentrations rise. This serves as the foundation for a quick test that can be performed to determine a potential blood donor high hemoglobin levels donating.

What does hemoglobin do?

Red blood cells include a protein called hemoglobin, which distributes oxygen to your body's tissues and organs while returning carbon dioxide to your lungs. A low red blood cell count indicates a low hemoglobin level, which can be determined by a hemoglobin test.

What is the remedy for low hemoglobin?

Iron supplementation and dietary changes are typically used as treatment for this type of anemia. Receiving iron thru a vein may be necessary for some persons. The source of the bleeding should be found, and the bleeding must be stopped, if blood loss—other than during menstruation—is the cause of iron deficiency.

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the system consists of the heart and blood vessels, which together move blood throughout the body. multiple choice question. pulmonary cardiopulmonary cardiovascular coronary

Answers

The blood and lymphatic vessels that circulate through the body make up the vascular system, also known as the circulatory system.

Explain about the circulatory system?

The circulatory system's blood arteries direct blood away from and toward the heart. The arteries carry blood away from the heart, and the veins carry it back. The circulatory system transports nutrients like oxygen and hormones to cells and removes wastes like carbon dioxide.

This system helps tissues get the right amount of nutrients, oxygen, and waste disposal. It is common to refer to the lymphatic system as a part of the circulatory system because it connects to the blood system.

The heart and lungs are located in the thorax, or chest cavity. After passing through the body via the heart, blood is oxygenated in the lungs.

supplying the body's tissues, especially the lungs, with food and oxygen. the coagulation of blood, which contains immune system-supporting cells and antibodies, to limit excessive blood loss.

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