When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and _____.

a. Forward and reverse primers
b. Forward or reverse primers
c. Forward and reverse probes
d. Forward or reverse probes

Answers

Answer 1

Answer:

b. Forward or reverse primers

Explanation:

Sanger sequencing is a technique of DNA sequencing based on the extension of DNA fragments with variable sizes terminated with dideoxynucleotides at the 3′ end. This technique was developed by Frederick Sanger in 1977. In Sanger sequencing, a short primer is added in order to bind by complementarity to the target DNA region of interest. Subsequently, a DNA polymerase adds nucleotides (A, T, C and G) in the 5'-3' direction. Finally, the extension of the DNA strand is stopped by adding dideoxynucleotides, which are nucleotide analogs (i.e., modified nucleotides) that act as DNA synthesis terminators.

Answer 2

When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and forward or reverse primers.

Sanger sequencing refers to a laboratory procedure that's used in the determination of DNA sequence by using dideoxynucleotides. Sanger sequencing is also known as dideoxy sequencing.

For Sanger sequencing to work, one needs two pieces of information that will be used to apply the strategy of sentence game to the sequencing of DNA. It should be noted that forward or reverse primers are required for the reaction.

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Related Questions

Predict: Based on your hypothesis, predict how changing the rabbit population will affect the other organisms at first. Write “Increase” or “Decrease” next to each “Prediction” in the table.

Answers

Answer: Increase the rabbit population

Explanation: Because then the rabbits can support the hungry wolves,bears,hawks,Ext. by feeding them.

The musculoskeletal system, a collaboration
between the muscular and skeletal systems,
works to provide which functions?

Answers

Answer:

D. Movement

Hope this helps :)

Which statement best describes the relationship between prophase and telophase?


The spindle fibers bring the chromosomes together in prophase then apart in telophase.

The spindle fibers pull the chromosomes apart in prophase then bring them together in telophase.

The nuclear membrane forms in prophase and breaks down in telophase.

The nuclear membrane forms in telophase and breaks down in prophase.
There are other ppl who have asked this question but the answers seem wrong because in my notes it says that the fourth one is correct

Answers

Yeah the fourth one is right, chromosomes don’t get pulled in prophase, that’s anaphase. And also I don’t even know when the spindle fibers glue back the chromosomes, they get separated in each individual cell and split.

What biome is home to large herbivores who travel in herds in order to evade predators. Some
animals, like the zebra, use their herd and stripes to confuse predators.?
A. Tundra
B. Estuary
C. Savannah
D. Desert

Answers

Savannah is home to large herbivores who travel in herds in order to evade predators.

What is Savannah known for?

Savannah is known for its rich history, stunning architecture, and vibrant culture. It is the oldest city in the U.S. state of Georgia and was originally founded in 1733 as a British colony. Savannah is home to numerous historical sites and monuments, such as the Cathedral of St. John the Baptist, the Owens-Thomas House, the Savannah Historic District, and the Telfair Academy of Arts and Sciences. It is also known for its lush parks, such as Forsyth Park, which is home to the iconic Forsyth Fountain. The city is also known for its vibrant arts and culture scene, featuring many galleries, theaters, and performing arts venues. Additionally, Savannah is known for its diverse cuisine, with an array of restaurants serving up classic Southern dishes, fresh seafood, and international fare.

The savannah biome is home to large herbivores such as zebras, giraffes, antelopes, wildebeests, and buffalo, who travel in herds to evade predators. The stripes of the zebra also help to confuse predators.

Therefore, option C Savannah is the correct answer.

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The biome that fits this description is the Savannah. It is characterized by grassy plains with scattered trees and is home to large herbivores like zebras, who use their herd and stripes as a defense mechanism against predators.

So, the correct answer is C (Savannah).

What are herbivores?

Herbivores are animals that primarily feed on plants, such as leaves, stems, roots, fruits, and seeds. They have specialized digestive systems that are adapted to break down the complex cellulose found in plant cell walls into usable nutrients, such as carbohydrates and proteins. Examples of herbivores include cows, horses, rabbits, deer, and many species of insects, such as butterflies and grasshoppers.

The biome that is home to large herbivores who travel in herds to evade predators and where some animals, like zebras, use their herd and stripes to confuse predators is the Savannah. This biome is characterized by grassy plains with scattered trees and is found in tropical regions. The savannah is home to many large herbivorous mammals such as elephants, giraffes, and antelopes, who form herds for protection against predators like lions, cheetahs, and hyenas. The stripes on zebras' coats help to confuse predators by making it difficult for them to pick out a single target from the herd.

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Some preganglionic neuron synapses with the cell body of the
postganglionic neuron in the
ganglion
These ganglia are linked together to form the
Name some of the tissue/organs are innervated by these neurons:
Other preganglionic neurons pass through the sympathetic trunk and
synapse with the postganglionic neuron anterior to the spinal cord in
ganglia.
The axons of these preganglionic neurons are called
nerves.
Name some of the tissue/organs are innervated by these neurons:
The sympathetic nervous system also send neurons directly from the
spinal cord to synapse onto the
gland.
This would cause the release of
into the
blood.

Answers

Answer:

The cell bodies of the preganglionic neurons are in the brainstem or spinal cord of the central nervous system (CNS). The cell bodies of the postganglionic neurons are in autonomic ganglia located peripherally. Axon terminal of preganglionic neurons synapse on dendrites and cell bodies of postganglionic neurons.

The cell bodies of the postganglionic neurons are in autonomic ganglia located peripherally. Axon terminal of preganglionic neurons synapse on dendrites and cell bodies of postganglionic neurons.

What is nervous system and its function?

The nervous system is the major controlling, regulatory, and communicating system in the body.

Thus, cell bodies of the postganglionic neurons.

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What do all living things have? Check all that apply.entify Characteristics Sharedngs

feelings

metabolism

genes

a nutritional diet

organisms

a limited life span

cells

Answers

Answer:organismen

Explanation:

dieren,mensen, iets die leeft

Answer:

Metabolism,  genes,  a limited life span, cells.

Explanation:

3)
As blood sugar levels rise in the body, insulin is released by the pancreas to lower the levels,
This process allows your body to maintain normal blood sugar levels and is called:
A
homeostasis
B)
cell membrane
C)
glycogen
D)
glucose

Answers

A: Homeostasis
Reason: cell membrane has nothing to do with question and C and D are terms for sugars

answer:glycoge

explanation: insulin and glucagon help maintain a state called homeostasis in which conditions inside the body remain steady. When blood sugar is too high, the pancreas secretes more insulin. When blood sugar levels drop, the pancreas releases glucagon to raise them.

this is all what I know

I hope it help you


Lack of growth, degradation of development is referred to as...

Answers

Answer:

abnormal development or growth especially of cells.

Explanation:

Lack of growth, degradation of development is referred to as...

Where does the oxygen and carbon dioxide get exchanged in the respiratory system?
Question 7 options:


bronchi


trachea


bronchioles


alveoli

Answers

Answer:

Alveoli

Explanation:

During gas exchange oxygen moves from the lungs to the bloodstream. At the same time carbon dioxide passes from the blood to the lungs. This happens in the lungs between the alveoli and a network of tiny blood vessels called capillaries, which are located in the walls of the alveoli.

alveoli ; the exchange where oxygen and carbon dioxide takes place

How are solids, liquids, and gases the same?

Answers

Answer:

they all take up a certain amount of space and they also have mass like every other thing thing in this world :)

Explanation:

By the time a cell has reached metaphase, all the following have occurred except...

Answers

Answer:

C. Sister chromatids separate

Explanation:

During interphase, which is composed of the G1 phase, S phase and G2 phase, the cell grows and replicates its DNA and some of its organelles. During the S phase, the genetic material is duplicated into two sister chromatids, and centrosomes, which are nucleated microtubules that interact with the chromosomes to form the mitotic spindle, are also replicated. Metaphase is the third phase of mitosis, where sister chromatids align in the middle of the cell before their separation into two daughter cells.

Which is the most sustainable ?

Answers

go with the upper ones

The process of antibody-dependent cellular cytotoxicity (ADCC) allows ______ cells to bind to antibody-coated host cells that may have viral proteins in their plasma membrane in order to kill them, inducing apoptosis and limiting viral spread.

Answers

Answer:

The right response is "Natural killer".

Explanation:

Natural killer cells constitute immune cells which could also identify and ultimately destroy distressed or damaged cells. This might also target cancerous as well as tumors. That alone reduces inflammation that lyses this same cell cycle rather than being amongst the very first cells towards prepare an inflammatory system, and even more so towards virus cells, participating including its destruction.

stage of photosynthesis that uses energy from the sun

A. Autotroph
B.potential energy
C. Stroma
D. Calvin cycle
E. Heterotroph
F. Light reactions
G. Chemical energy
H. Chlorophyll

Answers

The light reactions convert light into energy (ATP and NADHP) and the dark reactions

F. Light reactions

Answer:

C. Stroma

H. Chlorophyll

Explanation:

I think if not then it's F as well

Which statement describes an interaction between the biosphere and the atmosphere that is related to
photosynthesis?
During photosynthesis, plant roots take in water from soil.
During photosynthesis, plants take in carbon dioxide from the air.
Through photosynthesis, energy stored in plants is released into the air.
Through photosynthesis, energy stored in plants is transferred to humans who eat them.

Answers

Answer:

B.

Explanation:

During photosynthesis, plants take in carbon dioxide from the air.

What causes a mutation?
The translation of RNA into the language of amino acids
O The production of a complementary strand of DNA
O Frequent replication of DNA
O An error in DNA replication

Answers

Answer:

C

Explanation:

Sometimes cells make mistakes in copying their genetic information, causing mutations. Mutations can be irrelevant, or they can affect the way proteins are made and genes are expressed.

describe the evolution of homo sapiens from austropith ancestor and clarify the order in which distinctive human traits aaroses

Answers

Answer:Human evolution is the evolutionary process that led to the emergence of anatomically modern humans, beginning with the evolutionary history of primates—in particular genus Homo—and leading to the emergence of Homo sapiens as a distinct species of the hominid family, ... Human evolution from its first separation from the last common ancestor

Explanation:

Clarence made a diagram to compare codominance and incomplete dominance. Which label belongs in the area marked Y? Neither allele is dominant. An intermediate phenotype is expressed in offspring. One allele is dominant over the other. Both dominant alleles are fully expressed in offspring.

Answers

Answer:

both dominant alleles are fully expressed in offspring.      

Explanation:

I hope this helped

Answer:

both dominant alleles are fully expressed in offspring.      

Explanation:

What kind of land feature is shown at point on this topographic map?
A
B
O A. A lake
OB. A mountaintop
O c. A depression
O D. Aflat area

Answers

Answer: b a mountaintop

Explanation:

Answer:

a mountain top

Explanation:

What is the purpose of the chloroplast in autotrophs

Answers

Answer:

Chloroplast is the fundamentle organelle for photosynthesis as it contains chlorophyll that absorb light and provide energy for the manufacturing of food by the process of photosynthesis.

Explanation:

Please, I would be grateful for some help!!!
BRAINLIST IF ANSWER! 15 POINTS!!!!

Thank you!
:)

Answers

What is the question?

cells produce the energy they need to survive through the process of

A. Cellular respiration.
B. Cellular specilazation
C. Cellular differentiation.
D. Cellular transport

Answers

Answer:

A. Cellular Respiration

Explanation:

Cellular respiration is a set of metabolic reactions and processes that take place in the cells of organisms to convert chemical energy from oxygen molecules or nutrients into adenosine triphosphate (ATP), and then release waste products. Therefore they produce their energy.

Identify the important features of the pre-australopithecine Sahelanthropus tchadensis. Features of Sahelanthropus tchadensis

Answers

Answer:

prominent brow ridges      

Explanation:

Sahelanthropus tchadensis is believed to be one of the oldest and ancient known species of the family tree of humans. They lived on this planet around 6 to 7 billions years ago. They are the extinct species of Homininae. This fossils of this species were discovered and announced in the year 2002. Some of the important features of this species are :

-- they have prominent brow ridges

-- they were found in the Central America  

-- they lived during the time of human chimpanzee divergence

If a specific gene code for a polypeptide is 120 amino acids long, approximately how many nucleotides long is the mRNA that codes for this polypeptide?

Answers

Answer:

360 nucleotides

Explanation:

Each amino acid corresponds to a specific codon, a sequence of three DNA or RNA nucleotides.

If there are 120 amino acids and a sequence of three nucleotides corresponds to one amino acid, then 120 × 3 = 360 nucleotides.

Hope that helps.

Answer: 360

Explanation: 120 x 3 = 360.

Rhonda Runsalot is a 20 year old woman. She is running at VO2max. Her heart rate is 200 b/min. Her stroke volume is 100 ml blood/b. Her arterial oxygen content is 20 ml O2/100 ml blood. Her venous oxygen content is 6 ml O2/100 ml blood. What is her cardiac output?

Answers

Answer:

Cardiac output = 20 L/min

Explanation:

The cardiac output of a person refers to the volume of blood the heart pumps in a minute. The normal range for cardiac output is about 4 to 8 L/min. However, this can vary depending on the metabolic needs of the individual at any instant of time, for example, it is higher when exercising than  when resting.

Cardiac output is calculated from the product of the stroke volume in liters/min and the heart rate in beats/min

Cardiac output  = stroke volume * heart rate

For the individual who is running; stroke volume = 100 mL/beat or 0.1 L/beat

heart rate = 200 beats/min

Cardiac output =0.1 L/beat * 200 beats/min

Cardiac output = 20 L/min

1. What was found in the cave in the Dominican Republic?
in jurassic park

Answers

Answer:

Well preserved fossils of ancient animals.

Explanation:

Prehistoric remnants or fossils of certain animals, such as crocs, primates and bats, have also been discovered surprisingly well protected in the underwater galleries of the Dominican Republic. These caves are found within the Cotubanama Nature Reserve, and that the last four years have been a disappointment to wildlife conservation and animal care policies.

Which of the following would NOT stimulate/force you to
breathe?

A.increased plasma acidity

B. reduced plasma oxygen (O2)

C.increased plasma carbon dioxide (CO2)

D.increased plasma oxygen (O2)

Answers

Answer:

(C). Increased plasma carbon dioxide (Co2).

Explanation:

Edge

Compare and contrast a space telescope to a terrestrial (earth bound) telescope.

Answers

Answer: Space-based telescopes like Hubble get a much clearer view of the universe than most of their ground-based counterparts. Ground-based telescopes can't do the same, because the Earth's atmosphere absorbs a lot of the infrared and ultraviolet light that passes through it.

Explanation:

Which example is a body fossil?


A) shrimp burrow


B) claw print


C) dinosaur nest


D) leaf imprint

Answers

Answer:

claw print

Explanation:

Looking at the Punnett square, list the possible phenotypes and frequencies of
Martha and John's children? *
A
a
a
Аа
аа
а
Аа
аа
Your answer

Answers

Answer:

Phenotypes:

Aa with a frequency of 2

aa with a frequency of 2

The children have equal chances of having aa or Aa

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