To create a knockout mouse, after introducing to ES cells a DNA construct in which a specific gene is mutagenized by the insertion of a drug resistance marker, the cells Multiple Choice that have incorporated the transgene by homologous recombination grow in the presence of the drug and these are injected into a host blastocyst. that lack the transgene grow in the presence of the drug and these are injected into a host blastocyst. that have deleted the transgene grow in the presence of the drug and the nuclei are injected into a host blastocyst. that lack the transgene grow in the presence of the drug and the nuclei are used for nuclear transfer.

Answers

Answer 1
I’m not sure but if u get the answer I’ll like it
Answer 2

To create a knockout mouse, after introducing to ES cells a DNA construct in which a specific gene is mutagenized by the insertion of a drug resistance marker, the cells, that have incorporated the transgene by homologous recombination grow in the presence of the drug and these are injected into a host blastocyst. The correct option is A.

What is biological marker?

A biomarker is an objective measure of what is going on in a cell or organism at any given time. Biomarkers can serve as health-related early warning systems.

To create knockout mice, researchers insert artificial DNA into the chromosomes contained in the nuclei of ES cells using one of two methods.

Both methods are performed in vitro, that is, on cultured cells grown in a laboratory setting.

After introducing a DNA construct into ES cells that mutagenizes a specific gene by inserting a drug resistance marker, the cells that have incorporated the transgene via homologous recombination grow in the presence of the drug and are injected into a host blastocyst.

Thus, the correct option is A.

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Related Questions

In order to conserve resources, Evelyn uses her old homework as scrap paper
and never throws away paper with writing only on one side,
What kind of conservation is Evelyn practicing?
A. Recycling
B. Waste
C. Renewal
D. Reuse

Answers

Answer:

Evelyn is using reuse as a form of conservation

It’s reuse because she is reusing the paper by using the other side please mark me as brainliest

help i’ll give you brainilest

Answers

It’s the 3rd answer if ur going by the picture

A species of reef fish,living in ideal conditions,has a new generation of offspring every three months. In the new generation,approximately 200 new fish are added to the population of 10,000 fish.

Answers

Answer:

.02

Explanation:

Which statement accurately describes a phyroclsstic flow

Answers

Answer:

Pyroclastic flows, which consist of basaltic lava infused with incandescent ash and lava fragments, can reach many miles away from their sources because they are nearly frictionless and are driven by gravity.

Explanation:

which part control one direction transmission of impulses​

Answers

THE BRAIN FROM TOP TO BOTTOM. In a chemical synapse, a nerve impulse can travel in only one direction. In contrast, in an electrical synapse, the impulse travels in both directions.

Definition of respiration​

Answers

a process in living organisms involving the production of energy, typically with the intake of oxygen and the release of carbon dioxide from the oxidation of complex organic substances

Answer:

A process of oracajsjsjsjsjsjjss

Explanation:

Nithingbs lol just for poimts

What are the possible blood types of children in the following families?
(a) Type A mother, Type A father

(b) Type A mother, Type AB father

(c) Type AB mother, Type AB father

(d) Type A mother, Type B father

Answers

Answer:

a) A and O

b) A , B and AB

c) A, B and AB

d) A, B , O and AB

Explanation:

Select all the correct
Images.
Select the atomic models that belong to the same element.

Answers

There’s no image attached

Answer:

Theres no image

Explanation:

F-R-E-E P-O-I-N-T-S FOR EVERYONE

Answers

Answer:

K- T-H-A-N-K-Y-O-U

Explanation:

:))))

THANK Y-O-U-U-UUUUUUU

Letter A represents a wave's _____.
A. wave height
B. crest
C. wavelength
D. trough

Answers

I think that letter a should be C. Wavelength
i’ve heard this before and i think it’s wavelength

Explain how ocean temperatures affect the climate of coastal cities.

Answers

Answer:

Explanation: Warm ocean currents heat the air above the water and carry the warm air to the land, increasing the temperature of the coastal region. The same is true for cold ocean currents, except that they carry cooler air to the land and lower the temperatures of the coastal region.

Answer:

Warm and cold ocean currents can influence coastal climate, but only when local winds come in from the sea. Warm winds heat the air over the ocean, causing temperatures to rise over land. Cold currents will cool the air and add cooler temperatures to the land.

Explanation:

Flower color in the garden pea is due to two interacting genes, A and B. If either gene is recessive (aaB_, A_bb, or aabb) then the phenotype is white. A dominant allele must be present at both genes for flower color to be purple. After a testcross of an individual with a genotype of AaBb, what proportion of the progeny will have purple flowers

Answers

Answer:

4/16 = 1/4 = 25% of the progeny is expected to be purple (AaBb)

Explanation:

Available data:

Flower color in the garden pea is due to two interacting genes, A and BaaB_, A_bb, or aabb ⇒ white flower A dominant allele must be present at both genes for flower color to be purpleAABB, AaBb ⇒ purple flower

Cross:

Parentals) AaBb    x     aabb

Gametes) AB, Ab, aB, ab

                ab, ab, ab, ab

Punnett square)      AB         Ab       aB        ab

                  ab       AaBb    Aabb    aaBb   aabb

                  ab       AaBb    Aabb    aaBb   aabb

                  ab       AaBb    Aabb    aaBb   aabb

                  ab       AaBb    Aabb    aaBb   aabb

F1) Genotype:

4/16 = 1/4 = 25% of the porgeny is exected to be AaBb4/16 = 1/4 = 25% of the porgeny is exected to be Aabb4/16 = 1/4 = 25% of the porgeny is exected to be aaBb4/16 = 1/4 = 25% of the porgeny is exected to be aabb

    Phenotype

12/16 = 3/4 = 75% of the progeny is expected to be white(Aabb + aaBb + aabb)4/16 = 1/4 = 25% of the progeny is expected to be purple (AaBb)

How can friction be lessened?

A). Add less force
B). Add more force
C). increase the speed
D). add wheels

Answers

Answer:A.) add less force

Does a virus have DNA, RNA, or both to carry its genetic material

Answers

Answer:

it was one of either it depends so Both

Explanation:

Virus carries RNA
Explanation: took course

Why is this flower able to self-pollinate?
O A. It has a pistil and anthers.
O B. It has stamens and anthers.
OC. It has a pistil and an ovary.
OD. It has stamens and an ovary.

Answers

Answer:

B

Explanation:

Why are members of the Kingdom Plantae important to members of other kingdoms?

Answers

Answer:

C

Explanation:

Plants have the unique ability of photosynthesis. this gives off oxygen to all other organisms

For the human population to increase in size, what must exceed the death rate?
birth rate

mortality

limiting factors

carrying capacity

Answers

Answer:

carrying capicity

Explanation:

the carrying capacity is the amount of species or organisms that can live in a given environment without altercations. So the carryong capicity would have to increase so that more organisms can occupy the given space.

Answer: Birth Rate

Explanation:

Which is true about the tundra biome?
a. It receives less direct sunlight than any other biome on earth.
b. It has more trees than any other biome on earth.
c. It can be found at low elevations all over the earth.
d. It has more types of plants and animals than any other biome on earth.

Answers

Explanation:

The answer is A. It receives less direct sunlight.

Answer:

For the fisr question it is A it recives less direct sunlight than other biomes .

For the secound question it is B because the source is warmer.

Explanation:

i hope this helped

15) Study the food web, What conclusions can be drawn from analyzing this food web?
l. All the energy contained within the organisms in the web comes from the sun.
II. The energy from the caterpillar is transferred to the plant.
lll. The hawk is the top predator.
IV. Energy is transferred from organism to organism in this food web,
V. The bunny is the organism that harnesses the energy from the sun.



A) I only
B) III and IV only
C) I, III, and IV only
D) II, III, and V only​

Answers

Answer:

C) I, III, and IV only

Explanation:

l. All the energy contained within the organisms

lll. The hawk is the top predator.

IV. Energy is transferred from organism to organism in this food web,

Earthquakes occur along the San Andreas Fault. Which is an example of this type of plate boundary

Answers

Two of these moving plates meet in western California; the boundary between them is the San Andreas fault. The Pacific Plate (on the west) moves northwestward relative to the North American Plate (on the east), causing earthquakes along the fault.

Have a good day
Yeah I believe what the other person said

female reproductive system (side view)​

Answers

Answer:

.................................

Describe the pattern of inheritance that is most likely associated with a mutation in the MT-ND5 gene. Explain why individuals are not typically heterozygous with respect to mitochondrial genes.

Answers

Answer:

this gene is a mitochondrial gene and therefore it is maternally inherited

Explanation:

The MT-ND5 gene is a gene of the mitochondrial genome that provides instructions for synthesizing a protein called NADH dehydrogenase 5, which is part of the complex I (the first enzyme of the respiratory chain). In most multicellular organisms, the mitochondrial DNA (mtDNA) is inherited from the mother (i.e., maternally inherited), whereas nuclear DNA (i.e., nuclear genome) is inherited equally from both parents. For example, mitochondria in human sperm are usually destroyed after fertilization. In consequence, mitochondrial mutations are transmitted by maternal inheritance, and individuals, therefore, cannot be heterozygous for mitochondrial genes.

The pattern of inheritance that is most likely associated with a mutation in the MT-ND5 gene is

MT-ND5 is a mitochondrial genome coded gene responsible for the protein NADH-ubiquinone oxidoreductase chain V protein (ND5). ND5 is a sub-unit od NADH dehydrogenase, a mitochondrial protein. The mutant MT-ND5 will be transferred to the progeny via maternal mitochodral mtDNA.

Individuals are not typically heterozygous with respect to mitochondrial genes because

Heterozygousity of alleles of a particular gene arises due to contribution of both maternal and paternal genes. This is what happens for nuclear chromosomes. Mitochondria is an organelle that contains its independent genome. Only maternal mitochondria gets selected and thus are successfully transferred to the next generation post fertilization.

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a chemical reaction that removes electrons from an atom is call a blank reaction

Answers

Answer:

answer is b: oxidation reaction

Answer:

oxidation reaction

Explanation:

DNA and RNA are made of what 3 part structure?
1)nucleotide
2)codon
3)amino acid
4)nucleic acid

Answers

Answer: nucelotide

Explanation: Rna and dna are made up from nucelotides, they each have a five carbon sugar backbone, a nitrogen base and a phosphate group

What is the difference between genetic drift and natural selection?

Genetic drift is the random increase or decrease of certain genes in a population; natural
selection is not random.

Genetic drift is the selection of random traits in a population; natural selection is the
selection of specific traits.

Genetic drift is the frequency of genes in a population; natural selectic is the frequency
of adaptations in a population.

Genetic drift is the change in a population's genes over time; natural selection is the
evolution of a population over time.

Answers

A) Genetic drift is the random increase or decrease of genes in a population. Natural selection is not random.

Genetic drift is the change in the frequencies of alleles in a population due to chance events (random survival). Genetic drift exhibits strong effects in small populations and plays a smaller role the larger the population gets. Natural selection is the non-random change in the frequency of alleles in a population over time, because the change in frequency of the alleles is dictated by the different relative fitnesses that different alleles confer.

how the cardiovascular, respiratory and digestive systems work together to provide cells with the raw materials they need to ensure healthy cell function

Answers

Explanation:

The respiratory system works directly with the circulatory system to provide oxygen to the body. Oxygen taken in from the respiratory system moves into blood vessels that then circulate oxygen-rich blood to tissues and cells.

HELP!! 20 POINTS!!
Why is it more difficult for top carnivores to obtain sufficient energy resources than it is for animals lower on the food chain?

Answers

the trophic pyramid is the reason

what the trophic pyramid is:  Trophic pyramid, also called an energy pyramid, showing the progression of food energy. The pyramid base contains producers, organisms that make their own food from inorganic substances. All other organisms in the pyramid are consumers

- predators get little energy from the prey they eat so they must consume multiple prey

It is more difficult for top carnivores to obtain sufficient energy resources than it is for animals lower on the food chain because the higher trophic levels have less energy than the lower ones.

The trophic levels of food chain

The food chain is defined as a feeding pathway that consists of similar organisms that are placed in the same trophic levels.

Trophic levels in a food chain are those levels that is occupied by a group of organisms that have a similar feeding mode.

The plants which are the producers are at the lower trophic level while the carnivores are at the higher trophic levels.

The carnivores eats meat from other animals but not plant or plant products which obtain direct energy from sunlight.

Therefore, It is more difficult for top carnivores to obtain sufficient energy resources than it is for animals lower on the food chain because the higher trophic levels have less energy than the lower ones.

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Describe the preservation principle of salting ​

Answers

Salting, especially of meat, is an ancient preservation technique. The salt draws out moisture and creates an environment inhospitable to bacteria. If salted in cold weather (so that the meat does not spoil while the salt has time to take effect), salted meat can last for years.☜(゚ヮ゚☜)   (⌐■_■)

Why does the earth have different layers?

Answers

Answer:

The earth has different layers because as it formed, the lighter parts (like continental crust) floated to the surface, and the really heavy parts (like iron and nickel in the core) sank to the middle. It is just like when you mix oil and water: the oil will float to the surface because it is lighter (or less dense) than the water.

1 pc
Which structure does the following picture represent? *
Nitrogenous base
O Nucleotide
O Deoxyribose
Please no links ty

Answers

I’m pretty sure a nucleotide. Since it seems to be made up of a phosphate group, pentose sugar, and nitrogenous base. Hope this helps!
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Which of the below is the correct name for a plane that contains points A, B, C, and D?plane CDEplane CDplane ACDplane A How many atoms are in 2.2 moles of Zinc? A company acquires a natural resource for and spends another on development of the site and for a nonmovable tangible asset installed at the site and for tangible movable equipment. Both assets have an expected useful life of 10 years. The natural resource is expected to yield units over its expected life. In year 1, units are extracted from the resource. What is the depletion expense for year 1? (Round any intermediary calculations to the nearest cent, and round your final answer to the nearest dollar.) A. B. C. D. which of the following had the greater impact on America in the 1920s Suppose that you must go into court and testify as to the level of X in the river water. Your lawyer is concerned that your analytical results are lower than they should be because you cannot extract with benzene (distribution Ratio K=4) all of X from your samples. Assume the concentration of X in the 1.00 L river water sample is 1.06 x 10-4 M.a. How many extracts would be required to remove all but 1000 molecules of X from the sample with repeated 10.00 mL portions of extract?b. How many extractions are required for a 99.99% chance of extraction of the last molecule remaining (0.01% chance of a single molecule left behind) Which pollutant reduces the ability of the circulatory system to transport oxygen?A)particulate matterB)nitrogen oxideC)carbon monoxideD)sulfur dioxide Which of the following are ways that hormone levels can be controlled? (Choose all that apply) A) The heart B) Other hormones C) Body fluidD) Muscles E) The nervous system 3(m - n + 2) + 6 - m Choose the best selection for thequadrilateral with vertices at thefollowing points:(0,0), (0,3), (4,0), (4,3) ( PLEASE )why does to kill a mockingbird have such a grim conclusion. Here is the problem for you :) solve for the value of y Which of the following is NOT true about non-governmental organizationsa There are thousands of different examples all over the worldb They exist in both rich and poor countriesc They are tied to and funded by state governmentsd They support many different causes may someone help me please and thank you, how do u find an equivalent fraction?? :) A(n) _______ is a mapping that does not change the size of the original figure.A. IsometryB. MappingC. ImageD. Preimage PLEASE HELP ANSWER ASAP NO LINKS PLZ Select the correct answer.What does an individual's effective tax rate indicate?.the average Income earnedB.the average tax rate C.the minimum tax rateD.the next year's tax rate A box containing a present is shown.How much wrapping paper is needed to wrap the present?A. 41 in2B. 82 in2C. 11.5 in2D. 44 in2 Portia Grant is an employee who is paid monthly. For the month of January of the current year, she earned a total of 8,988. The FICA tax for social security is 6.2% of the first $128,400 of employee earnings each calendar year and the FICA tax rate for Medicare is 1.45% of all earnings. The FUTA tax rate of 0.6% and the SUTA tax rate of 5.4% are applied to the first $7,000 of an employee's pay. The amount of federal income tax withheld from her earnings was $1,491.37. Her net pay for the month is: (Round your intermediate calculations to two decimal places.) Multiple Choice $6,566.00 $6,809.04 $5,074.63 $7,366.30 $6,375.04 40% is NOT equivalent to....? A. 0.4 B.2/5 C.40/100 D. 0.04 E. None correct