The generation of a knock-out organism generally requires all the following EXCEPT ________. The generation of a knock-out organism generally requires all the following EXCEPT ________. knowledge of the sequence product embryonic stem cells knowledge of the target sequence a targeting vector

Answers

Answer 1

Answer:

knowledge of the sequence product

Explanation:

A gene knockout is a technique used in molecular genetics to deactivate target genes in an organism in order to study their functions by reverse genetics (i.e., gene loss). Knockouts are generated by different methods including, for example, homologous recombination or site-specific nucleases (zinc-fingers, TALENS, CRISPR/Cas9). These techniques require to know a priori the sequence of each gene to be knocked out in order to target desired mutations. In the last years, the CRISPR/Cas9 tool has gained attention to knockout genes of interest because it is a genome editing system that can be easily used for deletion or insertion of bases.


Related Questions

help and not just guess i need someone who actually knowz

Answers

Answer:

no

Explanation:

though the waves of the graph are same but it is not constant because the waves goes down and up again. we know constant is what goes without falling down or climbing up.

Porcupines have long sharp hairs called quills. What is the main function of the quills on the porcupine?

Answers

Answer:protection from other predators.

Explanation: the quills will stick into a predator that attack the porcupine Note: it does NOT shoot its quills

Answer: protection from other predators.

Which term refers to the fact that naturally occurs omg he universe

Answers

Answer:

Scientific law.

Explanation:

A scientific law is a statement that the scientific community elaborates on after observing a natural phenomenon and performing several experiments concerning certain phenomena. Scientific laws aim to explain the reasons why something happens and what may cause it to predict it in the future.

What effect does studying with music have on student test scores hypothesis?

Answers

Answer:

It helps them to focus

Explanation:

some people focus better when listening to music

Answer:

For some students it can help and for some it cant

Explanation:

Plants and animal cells are examples of
cells
O prokaryotic cells
O eukaryotic cell

Answers

Answer:

eukaryatic which the RH whitthakar was divided PLANTAE and animalia kingdoms in eukaryotes

The Hawaii Islands were formed as a result of
A. lava flowing over Earth's surface where two tectonic plates move apart
B. an oceanic plate moving over a mantle hot spot
C. two oceanic plates colliding to form an island arc
D. tectonic plates sliding past each other

Answers

The correct answer is A

Answer:

a is your answer

Explanation:

Which type of neurons keep us informed about what is happening both inside and outside of our bodies?

Answers

Answer:

Sensory neurons keep us informed of is happening both inside and outside our bodies.

Explanation:

How do plants contribute to the water cycle?

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Answer:

Plants absorb water from the soil. The water moves from the roots through the stems to the leaves. Once water reaches the leaves, some of it evaporates from the leaves, adding to the amount of water vapor in the air. This process of evaporation through plant leaves is called transpiration.

I really hope this helps!

Plants contribute to the water cycle in several ways through Transpiration, evaporation, Precipitation: and infiltration.

How do we explain?

Plants take in water through their roots and transport it up to their leaves. As water evaporates from the leaves, it creates a force that pulls more water up from the roots through a process called transpiration, and it is a major way that water moves through the atmosphere.

Water from plants and soil also evaporates into the atmosphere. This process is called evaporation, and it is another important way that water moves through the water cycle.

When water vapor in the atmosphere cools, it condenses and falls back to Earth as precipitation. This can be in the form of rain, snow, sleet, or hail.

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Is your prediction supported by the membrane potential chart? Explain your answer.

Answers

Answer:

Yes, it is. The chart shows that the initial charge of the neuron is negative. When the neuron is stimulated, sodium ions enter the cell. So, the voltage inside the cell changes to positive. When potassium ions move outward, the voltage decreases until it reaches its previous state.

Explanation:

Plato answer

Explain in a few sentences how the carbon, nitrogen, and phosphorus overlap. How do these cycles cross paths through earths spheres?

Answers

Answer:

Explanation:Energy flow and nutrient circulation are the major functions of the ecosystem.

Energy is lost as heat forever in terms of the usefulness of the system. On the other hand, nutrients of food matter never get used up. They can be recycled again and again indefinitely.

Carbon, hydrogen, oxygen, nitrogen and phosphorus as elements and compounds makeup 97% of the mass of our bodies and are more than 95% of the mass of all living organisms.

In addition to these, about 15 to 25 other elements are needed in some form for the survival and good health of plants and animals.

These elements or mineral nutrients are always in circulation moving from non-living to living and then back to the non-living components of the ecosystem in a more or less circular fashion.

This circular fashion is known as biogeochemical cycling (bio for living; geo for atmosphere).

Among the most important nutrient cycles are the carbon nutrient cycle and the nitrogen nutrient cycle.

There are many other nutrient cycles that are important in ecology, including a large number of trace mineral nutrient cycles.

How can the role of epigenetics in cancer be reconciled with the idea that cancer is caused by the accumulation of genetic mutations in tumor-suppressor genes and proto-oncogenes

Answers

Answer:

Epigenetic mechanisms control the activity of genes involved in DNA repair pathways and cell growth

Explanation:

Epigenetics can be defined as the study of the alterations in gene expression patterns that do not involve alterations in the DNA sequence. Epigenetic mechanisms include 1-DNA methylation, 2-histone modifications (i.e., methylation, acetylation, phosphorylation, ubiquitination, etc.) and 3-non-coding RNA pathways (microRNAs, long ncRNAs, etc). These epigenetic mechanisms work together to control gene expression patterns by activating or silencing genes at the level of transcription, in a mode that is dependent on the cell type and developmental stage. In consequence, the failure of the epigenetic mechanisms modifies the activity of genes which may be involved in DNA repair pathways and thereby it may lead to severe disorders including some cancers.

Why is it important that animals have different characteristics? Include at least two examples of animals and their characteristics.​

Answers

Answer:

some animals have 4 legs and some have 2

Explanation:

What two major events occur during meiosis that give the new cells genetic variation

Answers

Genetic variation is increased by meiosis

Because of recombination and independent assortment in meiosis, each gamete contains a different set of DNA. This produces a unique combination of genes in the resulting zygote. Recombination or crossing over occurs during prophase.

SOMEONE PLZ HELP ME, THIS IS DUE TODAY PLZ!!! I WILL MARK BRAINLIEST!!!!!!!!!

Answers

Answer: hi

Explanation:

If you were beamed into the Earth's outer core and you wanted to move to somewhere cooler you would:

Answers

No you don’t. Cooler by the way

The graph shows the predicted change in land usage when the population growth rate is positive and average. What effect would average population growth have on land usage?

Answers

The effect that the average population growth would have on land usage, considering the given graph is that: Option D.

What is Urban Land Use?

Urban land use can be described as the level of activities reflected by a location which involves the following:

RetailingManufacturingResidenceInstitutional and public buildingsTransportation, among others.

Urban land use increases, normally, as population increases. From the graph shown, the purple line shows urban land use increased above 30%.

Therefore, the effect that the average population growth would have on land usage, considering the given graph is that: Option D.

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Answer:

Urban use of land rises to more than 30 percent of available land

Explanation:

Which cellular structure in plant cells is most responsible for having less flexible animal cells

Answers

Answer:

The cellulose cell wall in plants

Explanation:

All eukaryotic cells, both plant and animals cells have a cell membrane which is an outer covering of the contents of the cell. Also plants and animals cells have many structures and cell organelles in common such as the mitochondria, the endoplasmic reticulum, ribosomes, golgi bodies, etc. However, plant cells have a more rigid structure than animals cells because of the presence of an extra structure known as the cell wall, covering their cell membranes.

The cell wall is a rigid layer that is found outside the cell membrane and surrounds the cell, providing structural support and protection. The cell wall is composed mainly of cellulose, but contains proteins as well as other polysaccharides too.

Another structure present in plants that serves to maintain the rigidity of the plant cell is the large central vacuole. The main role of the vacuole is to maintain turgor pressure by pushing the plasma membrane against the cell wall due to the pressure of fluids contained within it.

The Ebola virus can often have symptoms similar to bacterial pneumonia, an infection of the lungs. These symptoms include chest pains, shortness of breath, and fever. Which statement best summarizes the differences between these two diseases? (SC.6.L.14.6)a.Viruses can replicate because they are living organisms, while bacteria cannot. b.Viruses are not living organisms, but bacteria are living. c.Viruses can be beneficial to live organisms but bacteria are often infectious. d.Viruses are living organisms, while bacteria are not.

Answers

Answer:

Eblow is a terrible thing.

Explanation:

Answer:

I think its, option b) Viruses are not living organisms, but bacteria are living.

Explanation:

The other options don't fit "summarize the differences b/w bacteria and virus", or simply don't make sense...  

Bacteria can replicate as well, not only virus, so that cannot be a difference

The virus is often the one being more dangerous, there is something called "good bacteria"

A virus isn't  living, (for the sake of this answer), and we all know for sure that bacteria is living

So, that's how option b is correct

I hope this helped you!!

Which of the following is not a viral disease : (needed ASAP)

1. AIDS
2. Typhoid
3. Mumps
4. Polio ​

Answers

Answer:

It is typhoid. typhoid is not a viral disease

Answer:

Typhoid is not a viral disease.

Explanation:

Viral diseases are spread through virus particles, while typhoid is spread from typhi bacteria.

Which valve allows for the blood to be pumped into the aorta?

Answers

Answer:

Aortic Valve

Explanation:

I just know it

in an experiment the group is exsposed to the varible to be tesetd is called the

Answers

Answer: Experimental Group

Explanation: This group is being scientifically tested on and is exposed to the variable. Compared to a Control Group which doesn’t get scientifically tested on and isn’t exposed to the variable.

Hope this helps you, sweetie!

SOMEONE PLZ HELP ME!!!!!!!!!!!!!!!!!!!!!!

Answers

Plant cells!!!!!!!!!!!

Please someone help me with my Biology questions. PLEASE ​

Answers

Which Questions. I cant find them

Answer:

I cant see the question

Explanation:

Which division has a single preganglionic neurons with many axon collaterals that forms 20 or more synapses with postganglionic neurons?

a. sympathetic motor division
b. parasympathetic motor division
c. sympathetic sensory division
d. parasympathetic sensory division

Answers

Answer:

i believe its c

Explanation:

A single sympathetic preganglionic fiber has many axon collaterals and may synapse with 20 or more postganglionic neurons.

The postganglionic axons typically terminate in several visceral effectors and therefore the effects of sympathetic stimulation are more widespread than the effects of parasympathetic stimulation.

explain movement of water in the root​

Answers

Answer:

Water initially moves into the root hair cells by osmosis, because the mineral content of the cells is higher than that of the surrounding environment. Thus, a root pressure is established and extends into the microscopic tubes of the xylem.

Explanation:

We know gender differences are based in biology because all cultures practice the same gender norms and ideologies.




True

False

Answers

Answer:

false

Explanation:

not all cultures practice the same gender norms and ideologies

False❤️
It is what the other person said, please give them brainliest for being smart

Please select the word from the list that best fits the definition

Brings dry, clear weather

Answers

Answer:

conduction?

Explanation:

Answer:

anticyclone/2nd one down

what is the main function of nervous tissue? a: to transport materials and defend the body, b. send signals to control the body, c. contract the casue movement in the body, d. cover the body to protect other cells

Answers

Answer: to contract to cause movement in the body

Explanation:

AP*X

The main function of nervous tissue C. contract causes movement in the body.

What is the main function of the nervous system and how is it structured?

The nervous system is a complex collection of nerves and specialized cells known as neurons that transmit signals between different parts of the body. It is essentially the body's electrical wiring. Structurally, the nervous system has two components: the central nervous system and the peripheral nervous system.

Conclusion: The nervous system is a highly integrated system. The nervous system has three overlapping functions based on sensory input, integration, and motor output. At a more integrative level, the primary function of the nervous system is to control and communicate information throughout the body.

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The fruits have several purposes. Which purposes might be similar to those of a cone?

Answers

Answer:

Flowers and cones have different structures. Cones when mature have a woody texture and is made up of scales while flowers have colorful petals, sepals and both male and female reproductive portions are called stamens and pistils.

Explanation:

Answer:

the other side of a cone

Explanation:

Which of these is an environmental benefit of converting to solar energy?
O A. an increase in the use of fossil fuels
O B. a decrease in air pollution
O C. an increase in the cost of energy
O D. a decrease in electrical energy production

Answers

Answer:

i think it's the first one

The answer is B. a decrease in air pollution.

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True or False? Metamorphic rocks must remain solid during the process of metamorphism. URGENT!!! How was the colonial areas of America more successful than Latin American areas in creating a sound government and economic system? A company that makes hair-care products had 9,000 people try the new shampoo. Of the 9,000 people, 36 had a mild allergic reaction. Wht percent of the people had a mild mild allergic reaction? Nutted now Im shaking Prove that DE is parallel to BC. Please help, will award brainliest. Is Steel a compound? For f(x)=-5x+10, what is the value of x for which f(x) =35X = 5None are correct X=3X=-4X=-5 Who started the Kimberly Process and why? In timed pls help To find this product, Julissa first multiplied 83 x 49. 0.83 x 0.49 = 4067Where should she place a decimal point in the final product O Before the 4O after the 4 O after the 0O before the 7 Please Show Your Work All My Points Please Help Me!!!!! 6x^2+8x-6=2-2x-2 Which method is the best way to communicate about difficult issues?O by letterO over e-mailO in personO on the phone what were the main features of hitler's concept of lebensroum? Give five points Should I shade with makeup or pencil for the cloud drawing? Two friends argue over who brushes their teeth more often. To settle the argument, they keep track of the number of mornings and nights they brush and calculate a probability. These are shown in the table. Greek philosophers taught that A.every person is importantB.all life is preciousC.individuals should seek power and wealthD.you should question everything in order to understand the world Mitochondria are found within: A) All prokaryotic cells B) A cell's plasma membrane C) A cell's nucleus D) Eukaryotic cells E) None of these answers are correct Why did Dunbar use "Negro Dialect" in his poetry? B) A travs de su criterio, describa las razones que pueden llevar a la humanidad a cometer estos crmenes tan atroces. en Formas de dominio y aniquilacin Espaola contra los aborgenes americanos What was the long term economic effects of the Punic Wars on Rome? This elementary problem begins to explore propagation delay and transmis- sion delay, two central concepts in data networking. Consider two hosts, A and B, connected by a single link of rate R bps. Suppose that the two hosts are separated by m meters, and suppose the propagation speed along the link is s meters/sec. Host A is to send a packet of size L bits to Host B a. Express the propagation delay, dprops in terms of m and s b. Determine the transmission time of the packet, drans, in terms of L and R. c. Ignoring processing and queuing delays, obtain an expression for the end- d. Suppose Host A begins to transmit the packet at time t = 0. At time t = dtrans' e. Suppose drop is greater than dran . At time t = d, ans, where is the first bit of f. Suppose dprop is less than dtrans. At time t = dtrans, where is the first bit of g. Suppose s = 2.5-108, L = 120 bits, and R = 56 kbps. Find the distance m so that dprop equals drans