in the "sandwich" elisa technique, what is the function of the capture antibody?

Answers

Answer 1

Answer:

To concentrate the molecule of interest.

Explanation:

Happy Thnskgiving!

Answer 2

The "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is ​​rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.

What is Elisa Technique?

ELISA stands for enzyme-linked immunosorbent assay. It is a commonly used analytical biochemistry assay. The assay uses a solid-phase type enzyme immunoassay to detect the presence of a ligand in a liquid sample using antibodies directed against the protein to be measured.

It is a plate-based assay technique designed for the detection and quantification of soluble substances such as peptides, proteins, antibodies and hormones. The antigen, called a target macromolecule, is immobilized on a solid surface called a microplate and then complexed with an antibody that is bound to a reporter enzyme.

There are many types of ELISA such as Sandwich ELISA. It measures the antigen between two layers of antibodies called capture and detection antibodies. The target antigen must have at least two antigenic sites capable of binding to the antibody

Thus, the "capture" antibody binds to and retains the analyte from the sample while the rest of the matrix is ​​rinsed away. An enzyme-conjugated "detector" antibody, raised against a different epitope upon analysis, is added to the plate.

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Related Questions

if the cerebrum was no longer able to communicate with the midbrain, what type of tract would you suspect has been damaged?

Answers

The projection tracts(projection fibers )  was severed. Because it is one of the the links between the cerebrum and the midbrain.

Commissural tracts and Association tracts are other type of bundle of axon in the brain.

The bundle of axon fibers (white matter tact) that  connects the cerebrum wit other parts of the Central Nervous System is called the projection tracts.

Anatomically, these tracts project vertically  between the higher brain ( the medulla oblongata, pons and the midbrain) and lower brain centers(brain stem, spinal cord and the cerebellum) to serve as the conduit for information transfer between the cerebrum and other parts of the CNS. Examples are: Corticospinal tracts, Corticobulbar, medial Lemniscus and more

Therefore if there is loss of communication between the cerebrum and the midbrain ,the tract is damaged.

It can not be either of Commissural tracts and Association tracts because the former enables the connection between the two cerebral hemispheres, and the later connect the memory center of the brain with the perceptual.

Therefore the answer is projection tracts

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What effect will most likely result from a combination of drug abuse and unprotected sex with different partners?

increased risk of developing a sexually transmitted infection
decreased chance of developing cervical cancer
decreased risk of developing HIV infection
increased risk of unplanned pregnancy

Answers

Answer:

Increased risk of developing a sexually transmitted infection

Explanation:

Drug abuse typically means that the person would most likely be under influence, and therefore cannot think properly during that set duration.

Unprotected sex typically means that there is no protection at all from STDs if one or both of the partners have STDs currently.

Different partners means that there is a higher chance of obtaining STDs from one or more of the partners.

Therefore, your answer should be increased risk of developing a STD.

Answer:

(a) is the answer

Explanation:

took test on edge

Why do u get sick after getting vaccinated

Answers

Answer:

your imune system is responding to the illness

Explanation:

what hormones are released that cause you to feel full after eating

Answers

Leptin. Leptin is produced by your fat cells. It's considered a “satiety hormone” that reduces appetite and makes you feel full. As a signaling hormone, its role is to communicate with the hypothalamus, the portion of your brain that regulates appetite and food intake.

how to get antibiotics for uti without seeing a doctor?

Answers

Answer:

you can't! you can always try a probiotic, along with cranberry juice but those are preventives. please seek medical  treatment. untreated UTI's can cause more problems in the body.

Explanation:

pregnant women, who use tobacco products can cause which health effect in their baby?

Answers

Answer: Smoking during pregnancy increases the risk of health problems for developing babies, including preterm birth, low birth weight, and birth defects of the mouth and lip. Smoking during and after pregnancy also increases the risk of sudden infant death syndrome (SIDS).

Answer:   Smoking during pregnancy can cause tissue damage in the unborn baby, particularly in the lung and brain.

the plexuses that lie on either side of the neck are the _____ plexuses.

Answers

Cervical plexus I believe

how long after hitting head can concussion symptoms start?

Answers

Answer:

Symptoms may present 24 to 48 hours after an injury. From my experiences symptoms for a concussion can start only a few minutes after the injury or a couple of hours after the injury.

Explanation:

I hope this helps!

24 hours to 48 hours it depends

true or false By definition, a socially healthy person has access to clean water and adequate living space and makes an effort to recycle.

Answers

[tex] \: \: \: \: \: [/tex]

TRUE

so our body is clean and were safe if we have clean body

hope it helps

[tex] \: \: \: \: \: \: \: \: \: [/tex]

That is very true , I would think so ..

Match the scenario to the type of mental healthcare barrier it describes.

Raj lost his job and no longer has insurance, so he is no longer filling his prescription for antidepressants.

Emily feels she needs a different dose after six weeks on her bipolar medication, so she stops taking the current dose and switches over to a different medicine on her own without consulting her doctor.

Rafael does not seek treatment for his obsessive-compulsive disorder because his community considers that a weakness.

Demetrius has been feeling anxious for the past two months, but he tells himself it’s just because there’s a lot going on at school.

.1denial

2.lack of funding

3.community stereotypes

4.self-medication

Answers

Raj has lack of funding

Emily is self medicating

Rafael is dealing with community stereotypes

Demetrius is in denial

what is the most common cause to food born illnesses?

Answers

Salmonella is the most common

describe the sequence of injuries that may occur if the extended, weight-bearing knee receives a very strong blow to the lateral side of the knee?

Answers

Answer:

A strong blow to the lateral side of the extended knee will cause the medial side of the knee joint to open, resulting in a sequence of three injuries. First will be damage to the tibial collateral ligament. Since the medial meniscus is attached to the tibial collateral ligament, the meniscus is also injured. The third structure injured would be the anterior cruciate ligament.

Explanation:

I hope this helped!

The USDA recommends choosing meats that are at least what percentage lean?
O 10% lean
O 50% lean
0 70% lean
O 90% lean
Pls answer
And if you have the whole test answers pls be nice and give out

Answers

It is d 90℅ lean

Explanation:

The USDA recommends choosing meats that are at least 90% percentage lean. Hence, option D is correct.

What is lean meat?

Meats having a low-fat content are referred to as lean meats. Lean meat options include skinless chicken, skinless turkey, and red meat like pork chops with the fat removed. They have high protein content and low calories.

Fish, chicken, and turkey will generally be your leanest meat options. These decisions might become even healthier with smart buying. (Poultry) Chicken and turkey Try to stick with the breast because the black meat and skin are the fattiest parts. Some examples of lean meat are beef, lamb, pork, kangaroo, etc.

The USDA recommends that meat that is 90% lean should be selected. Therefore, option D is correct.

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a patient is on a mechanical soft diet. which food item choice by the patient will cause the nurse to intervene?

Answers

Given our understanding of this diet type and the reasons behind it, we can confirm that a food item choice by the patient that will cause the nurse to intervene is red meat.

A mechanical soft diet is one in which the patient is allowed to eat solid food, however, the food must be soft and require a minimal amount of chewing. This diet is recommended for patients that have any form of digestive tract illness that may cause difficulty when chewing or swallowing food.

Red meat would cause the nurse to intervene as it presents a great risk for choking or other complications and is prohibited by this diet.

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what is the substrate for a antigen and b antigen synthesis?

Answers

Answer:

The blood group A antigen is synthesized by an N-acetylgalactosaminyltransferase that uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate, while the B antigen is synthesized by a galactosyltransferase that uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

The substrate for A antigen is N-acetylgalactosaminyltransferase which uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate and the substrate for B Antigen is galactosyltransferase which uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

What are Antigens?

Antigen is any substance which is recognized as foreign particle by the immune system. It is also known as Immunogens. It triggers the Immune system. Antigens can be endogenous, exogenous or autoantigens. Some examples of antigens are pollen, bacteria, viruses, etc.

There are three types of antigens which includes

1. Exogenous antigen - It is foreign to the immune system

2. Endogenous antigen- It is produced by intracellular bacteria and virus which replicates inside the host cell.

3. Autoantigens- It is produced by the host.

Antigen-presenting cells help the immune system triggers its attack. There are three types of antigen-presenting cells in your body. They are

1. Macrophages

2. Dendritic cells

3. B cells

Thus, the substrate for A antigen is N-acetylgalactosaminyltransferase which uses a UDP-α-N-acetylgalactosamine (UDP-GalNAc) as sugar donor substrate and the substrate for B Antigen is galactosyltransferase which uses a UDP-α-galactose (UDP-Gal) as a sugar donor substrate.

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which part of the brain is not fully developed in adolescence?

Answers

Answer:

One of the biggest differences researchers have found between adults and adolescents is the pre-frontal cortex. This part of the brain is still developing in teens and doesn't complete its growth until approximately early to mid 20's.

Explanation:

how can teenagers help combat the opioid and heroin epidemic?

Answers

Answer:

It would be useful if they talked to somebody about the problem or see a therapist.

Talk to somebody and seek help! If you know anyone who is struggling with opioids and heroin, talk to them and point them in the right direction!

Signal Detection Theory.

What's a personal experience you've had with this?
Easy pts.! Feel free to make it up if you want :)
It's for my Psychology class and I don't know wt to write lol
I will give Brainliest too

Answers

My family from [INSERT PLACE] had flown in to visit and I was expecting them to knock any minute. As I was waiting for them, I began to “hear” them knock and when I opened the door nobody was there. I was “detecting” a stimulus, or signal, that was not there because it would be worse to miss them than to check to see if they were there, only to find that my family had not yet arrived.

you realize that many of your geriatric patients have visual problems that impact their care. which item is not a common cause of decreased visual acuity in the elderly?

Answers

Answer:

Abnormal production of vitreous humor.

Explanation:

I hope this helped!

how long does it take for swelling to go down after wisdom teeth removal?

Answers

Answer:

2-3 days

Explanation:

And stiffness and soreness will go away in 7-10

2-4 days for the swelling to go down

Select the skills or techniques being exhibited by the healthcare professional below. (Check all of the boxes that apply)


A young patient was just told that he has mononucleosis, or mono. The patient suspected he had it, so he looked it up on the Internet but did not learn much. He has no idea, for instance, where his spleen is – one of the parts of his body the doctor said was affected. The doctor points to the appropriate section on the chart of the human body on the wall, and gives the patient a sheet designed for teens about the illness, including what he should and should not do for the next month or so.


A. using visual aids to convey information


B. good Internet searching skills


C. take-home patient information


D. patient information specially designed for children

(it's A and C)

Answers

Explanation:

A. using visual aids to convey information

B. good Internet searching skills

C. take-home patient information

D. patient information specially designed for children

Answer:

your answer is A,C,and D

Explanation:

Cuz i said so

why do you exhale carbon dioxide and where does it come from?

Answers


We breathe in oxygen and some of this carbon dioxide. When we exhale, we breathe out less oxygen but more carbon dioxide than we inhale. The carbon we breathe out as carbon dioxide comes from The carbon in the food we eat.
https://www.smh.com.au › ... › Books
We breath in ox

Answer:

We breathe in oxygen and some of this carbon dioxide. When we exhale, we breathe out less oxygen but more carbon dioxide than we inhale. The carbon we breathe out as carbon dioxide comes from the carbon in the food we eat

Explanation:

TIME REMAININ
59:55
In general, a family can be defined as a group of
A individuals who live together and care for each other
B. individuals who share one car or vehicle
C individuals who enjoy each other's company
Please select the best answer from the choices provided.
Ο Α
ОВ
Mark this and return
Save and Exit
Next
Submit

Answers

I think the answers a

Answer:

A

Explanation:

C, could be anyone, your neighbours or even your teachers.

D, is more for friends than family.

administration is the placement of medication under the tongue where it is allowed to dissolve slowly.

Answers

Answer:

Sublingual

Explanation:

Sublingual Administration

The most beautiful contestant won the pageant. ""Beautiful"" has a negative connotation. Please select the best answer from the choices provided T F.

Answers

Answer:

I believe this would be false

The connotation of a word shows the context in which it is used. The word beautiful does not have a negative connotation hence the statement is false.

Connotative meaning

The connotative meaning of a word refers to the meaning that the word could have as derived from the context in which it is used. It is different from the denotative meaning of the word.

In this case, the word beautiful does not have a negative connotation hence the statement is false.

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is social media use linked to increase in anxiety, depression and other related mental illness?

Answers

Sometimes you might see things on social media that will give you anxiety, depression and other because some people on social media say random stories that aren’t true but you might take it seriously and start to ask random questions to yourself to increase your level of stress and anxiety, depression, etc.

Considering that overpopulation is a national concer, what is your take regarding unplanned pregnancies among young adults? How do you ascertain if one is ready to start a family?​

Answers

Answer:

Eighty-six percent of pregnancies and you're both excited and eager to start a family, and you're feeling settled financially

Explanation:

which use of protein for exercise is accurately described?

Answers

Answer:

It repairs muscle tissue after exercise.

Explanation:

The largest muscles in the body are your ______

Quads

Calves

Glutes

Hamstrings

Answers

The largest muscles in the body are your glutes

This tennis grip is obtained by laying the racquet flat and picking it up.

continental grip

western grip

eastern grip

double handed grip​

Answers

Answer:

Explanation:

western

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