Efficient production of eukaryotic proteins in E. coli may require all of the following except?
a.altered codon usage within the heterologous sequences to approximate the codon bias in E. coli
b.an E. coli expression vector with a Shine- Dalgarno sequence for efficient translation
c.a negative selectable marker to select against nonhomologous recombination events
d.The use of cDNAs as eukaryotic transgenes an E. coli expression vector with a promoter sequence that binds RNA polymerase D

Answers

Answer 1

(C). A negative marker to select against nonhomologous recombination events Efficient production of eukaryotic proteins in E. coli.

E. coli can it produce eukaryotic proteins?

The restricted function of E. coli's eukaryotic post-translational machinery is seen as a major hindrance to the production of eukaryotic phosphoproteins, such as serine/threonine/tyrosine protein kinases.

Which proteins are produced by E. coli?

The organism One of the most popular hosts for the production of recombinant proteins is Escherichia coli (Rosano and Ceccarelli, 2014). Even though E. coli's cytoplasm is where the majority of recombinant proteins are made, the periplasm is frequently where disulfide-containing recombinant proteins are made.

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Related Questions

What would be the consequence of ectopically expressing Hoxd10 throughout the developing mouse limb bud?

Check all that apply.

converting the thumb into a middle finger.
converting the thumb into an index finger.
converting the index finger into a middle finger.
converting the index finger into a thumb.
converting the middle finger into an index finger.
converting the middle finger into a thumb.

Answers

Converting the thumb into an index finger will be the correct option.

What is ectopic pregnancy?

In an ectopic pregnancy, a fertilized egg implants outside the uterus, usually in one of the fallopian tubes.

Fallopian tubes are tubes that connect the ovaries and the uterus. If the egg gets stuck inside, it cannot develop into a baby and can be detrimental to your health if the pregnancy continues. It usually needs to be removed medically or surgically.

About 1 in 90 pregnancies in the UK are ectopic. This equates to approximately 11,000 pregnancies per year.

Therefore, Converting the thumb into an index finger will be the correct option.

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A symbiotic relationship that benefits both species is referred to as commensalism. true or fales

Answers

Answer:

True

Explanation:

During skeletal muscle contraction, ____________ move closer together.
Question options:

A) H band

B) I band

C) Z disk

D) M line

Answers

Answer: C) Z disk

Explanation:

The temporary release of stress hormones into the bloodstream facilitates

memory reconstruction
repression
retrograde amnesia
retroactive interference
long-term potentiation

Answers

The temporary release of stress hormones into the bloodstream facilitates long-term potentiation (Option 5).

What is the process of long-term potentiation?

The process of long-term potentiation is a physiological process associated with the increase in the synaptic signaling pathway among nerve (neuronal) cells, which may be associated with the secretion of different chemical messengers including hormones.

Therefore, with this data, we can see that the process of long-term potentiation is associated with enhanced synaptic signaling and long-lasting neuronal communication, a process linked to the release of chemical messengers called hormones.

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ill in the blank. a gujarati village comprises a(n) of organisms: a. ecosystem b. habitat c. niche d. community

Answers

A Gujarati village comprises an ecosystem of organisms.

A typical Gujarati village consists of clusters of houses along a central street. The village center has a temple village square shops and a fountain. The land and trade castes live in this central area while the artisan castes live further afield.

An ecosystem is a community of organisms in a particular area. The term eco refers to a part of the world and the system refers to a coordinating entity. Gujarati, like other Indo-Aryan languages, was developed through Prakrit, a large group of ancient Indic languages, and Ababramsha, a transitional dialect spoken in India between the 6th and 13th centuries AD. It comes from Sanskrit.

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A species that has a large effect on the types and abundance of other species in an ecosystem

Answers

In an ecosystem, keystone species play important functions that have a significant impact on the kinds and abundance of other species.

What is meant by ecosystem?

An ecosystem is a region where a bubble of life is created by plants, animals, and other species interacting with the weather, environment, and other factors.

What role does the ecology play?

Human wellbeing and survival depend on healthy terrestrial ecosystems because they give us access to critical goods and advantages. More than 90% of our food originates from terrestrial ecosystems, which also offer us energy, resources for construction and clothing, health-related products, clean water, and pure air.

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After some important cellular processes, there is an abundance of carbon dioxide (co2) molecules inside the cell, but not enough molecules outside the cell. Co2 molecules would.

Answers

After some important cellular processes, there is an abundance of carbon dioxide (CO₂) molecules inside the cell, but not enough molecules outside the cell. CO₂ molecules would move outside the cell due to the process of diffusion.  

Cell is the basic fundamental unit of life that acts as the building block of all the organisms. A cell can be of two types based on its origin: prokaryotic and eukaryotic.

Diffusion is the process of movement of solute particles from the region of its higher concentration to lower concentration. This movement does not required the expenditure of energy and hence it is a passive process.

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the process of breaking down glycogen into glucose is called , while the process of synthesizing new glucose from other substrates is called .

Answers

Glycogenolysis is the process of converting glycogen into glucose, whereas gluconeogensis is the process of creating new glucose from other substrates.

Glycogenolysis is the biological process through which glycogen breaks down into glucose and glucose-1-phosphate. Both myocytes and hepatocytes take part in the reaction. Your body turns glucose into glycogen through a process called glycogenesis. Your body breaks down glycogen for use through a process called glycogenolysis. Those two actions Several enzymes participate in glycogenolysis. Gluconeogensis is the process by which glucose is created from non-carbohydrate metabolites. The main precursors for gluconeogensis are pyruvate, lactate, a few gluconeogenic amino acids, and glycerol, which is primarily produced by fat metabolism.

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Which two factors can both cause a population to increase?
1. birth rate and emigration
2. birth rate and immigration
3. death rate and immigration
4. death rate and emigration

Answers

Answer:

2

Explanation: birth rate and immigration since when people immigrate to an area, the population increases. When people are born, the population increases.

answer is 2 birth rate and immigration

how much physical factors such as temperature and salinity affect organisms is determined by the: group of answer choices environmental resistance carrying capacity ability to osmoregulate range of tolerance of the organisms

Answers

Physical factors such as temperature and salinity affect organisms is determined by the carrying capacity.

The physical and biological world where we live in - Environment. Each level of food chain where transfer of energy takes place - Trophic level. The physical factors like temperature, rainfall, wind and soil of an ecosystem - Abiotic factors. One of the most important abiotic factors influencing species composition in marine environments is salinity. Low-salinity (hypoosmotic) stress, which is common in many estuaries and coastal areas, can cause severe physiological stress in marine animals, affecting survival, immune responses, metabolism, protein function, and other cellular processes. Under moderate hypoosmotic stress, increased osmoregulation costs are met by increasing the routine metabolic rate or re-allocating energy to osmoregulatory processes. This, however, limits the potential for growth and reproduction, as well as, ultimately, fitness. The depletion of the organic osmolyte pool caused by low salinity has been proposed to set a critical boundary in osmoconforming marine invertebrates.

Desalination has been predicted for many coastal areas as a result of ongoing climate change, so it is critical to gain a better understanding of the mechanisms that limit salinity acclimation ability.

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What does the replication of DNA during interphase ensure during cell replication?

A. Mutations are connected before the cell divides

B. Each daughter cell gets an exact copy of the cells DNA

C. Crossing over occurs prior to cell division

D. Genetic variation is created in daughter cells

Answers

It would be B!!!! good luck!!

examine the phylogenetic tree showing the evolutionarily relationships of five organisms which statement most accurately explains the relationship between organisms and the other organisms 

Answers

A phylogenetic analyses is a visual representation of how different creatures have evolved over time. Phylogenetic trees are theories, not proven truths.

What in biology does phylogeny mean?

A phylogenetic tree, sometimes called a phylogeny, is a visual representation of how several species, creatures, or genes have descended over time from a single common ancestor.

What purposes does phylogeny serve?

A potent method for determining how modern species have evolved is phylogenetics. Scientists can better comprehend how species have developed while also gaining insight into the differences and similarities between various species by examining phylogenetic trees.

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n pea plants, the allele for purple flowers, p, is dominant over the allele for white flowers, p. which conclusion about phenotype and genotype is possible?(1 point)

Answers

Dominant and recessive are used in the inheritance pattern of traits. Dominant traits mask the expression of recessive alleles whereas recessive traits can be expressed when two copies of allele is present.

What is the conclusion about phenotype and genotype?

It is given that purple flower is dominant and white flower is recessive in pea plants. Hence, presence of at least one P allele will give purple color (dominant phenotype) to the flower. Therefore, the PP is a homozygous dominant genotype. Hence, the phenotype of PP is purple color.

Genotype of the purple (phenotype) flower is PP and that of white flower (phenotype) is pp.

As the purple flowers are dominant over the recessive, their genotype would be PP or Pp. White flowers phenotype would be white and genotype pp because they are recessive.

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Construct a food chain. Label the producer, primary consumer, secondary consumer, and tertiary consumer. An owl eats a snake, the snake eats a squirrel, the squirrel ate a nut.

Answers

In the example, the producer is a nut, the primary consumer is a squirrel, the secondary consumer is a snake, and the third consumer is an owl.

What is the consumer in biology?

Any organism that is unable to produce its own nourishment is a consumer. Consumers must eat from producers or other consumers in order to exist. Because they are herbivores, deer can only consume grass (Producers). A bear is another example of a consumer. 1. One who purchases products or services for personal use or possession as opposed to selling or use in production or manufacturing. 2. A heterotrophic organism in a food chain that consumes other organisms or organic substances.

Which organism is the consumer?

The organisms that eat the producers are the principal consumers. They are many and frequently little in size. Herbivorous animals are the main consumers (vegetarians). The organisms known as secondary consumers are carnivores that consume the prime consumers.

Briefing:

As a result, the food chain in this instance is nut—>squirrel—>snake—>owl.

According to the scenario, a nut is indeed the producer, the squirrels is the main customer, the snake is indeed the secondary customer, and the owl is the third consumer.

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Action potentials from receptors involved in general sensations are interpreted in the.

Answers

mesencephalon!!I believe

write the name of any two memberanes that completely cover the brain​

Answers

Answer:

the inner layer is pia mater

the middle layer is the arachnoid

the tough outer layer is dura mater

Explanation:

Answer:

Three layers of membranes known as meninges protect the brain and spinal cord. The delicate inner layer is the pia mater. The middle layer is the arachnoid, a web-like structure filled with fluid that cushions the brain. The tough outer layer is called the dura mater.

what is the correct pairing of neurotransmitters in the parasympathetic division of the autonomic nervous system?

Answers

The correct pairing of neurotransmitters in the parasympathetic division of the autonomic nervous system is that both release ACH.

What is ACH?


Acetylcholine inside the autonomic nervous system. The neurotransmitter in preganglionic sympathetic and parasympathetic neurons of the autonomic nervous system is called acetylcholine (ACh).

The heart, lungs, upper gastrointestinal tract, and sweat glands all have muscarinic acetylcholine receptors on their peripheral nerve systems.

The central and peripheral nervous systems depend on acetylcholine, a sort of chemical messenger or neurotransmitter, to function properly. It aids in learning, memory, and attention as well as the control of muscular motion and autonomic bodily processes.

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the theory that those who adapt best to the environment will have a greater chance of surviving and passing on their genes is known as ...

Answers

The theory that those who adapt best to the environment will have a greater chance of surviving and passing on their genes is known as Natural selection.

Natural selection is one process of evolution. Creatures with more environmental adaptation have a better chance of surviving and passing on the genes that gave them an advantage. This process causes species to change and diverge throughout time.

Natural selection is a technique used to adapt and transform populations of living things. People in a population are naturally diverse, which means that every person is distinctive in some ways. Due to this diversity, some people have traits that better suit their environment than others. One of the most important aspects of evolution is the modification of a population's inherited traits over successive generations.

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why do organisms that perform photosynthesis require water and sun light?

Answers

Answer:

Explanation:

Photosynthetic organisms need light because this is the source of the energy that is transformed to chemical energy by chlorophyll. These organisms need water because the electrons that are excited in chlorophyll and passed to the electron transport system are replaced with electrons from a split water molecule. Hydrogen is used to form the proton gradient across the thylakoid membrane. The electrons take from the oxygen molecules in water release an oxygen gas into the atmosphere which makes living possible for us.

All plant cell walls are made of _____ while all fungi cell walls are made of _____.

Answers

The cellulose and chitin-based cell walls of plants and fungus, as well as the cell walls of bacteria, are not the same.

What types of tactile sensors are there?

Merkel's disks, Meissner's corpuscles, Ruffini endings, and Pacinian corpuscles are the four main subtypes of tactile mechanoreceptors. In the upper layers of skin that has hair or is glabrous, Merkel's disks are slow-adapting, unencapsulated nerve terminals that respond to gentle touch.

What role does tactile play?

The tactile corpuscle is in charge of delivering low-frequency vibrations and light touch sensations from the peripheral nervous system to the brain.

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Prokaryotes that use light for their energy source and co2 for their carbon source are called _____.

Answers

Answer:

Photoautotrophs

Explanation:

Photoautotrophs are cells that capture light energy, and use carbon dioxide as their carbon source. There are many photoautotrophic prokaryotes, which include cyanobacteria. Photoautotrophic prokaryotes use similar compounds to those of plants to trap light energy.

What process of sexual reproduction do some but not all bacteria use?(1 point)
Responses

A. cloning

B. binary fission

C. endospore formation

D, conjugation

Answers

Answer: D

Explanation:

An enzyme in potato cell caue oxygen to be produced from hydrogen peroxide. Cube of potato were incubated with hydrogen peroxide at different temperature. The number of bubble of oxygen releaed per minute were counted at each temperature. The table how the reult. Temperature/°C 15 25 35 45 55
number of bubble / bubble per minute 96 98 82 36 1
The reult ugget the optimum temperature for the enzyme i between which two value

Answers

Between 45 and 55 °C is where the enzyme functions best.

What's the ideal temperature for an enzyme?

The majority of enzymes work best when the temperature is around 98.6 degrees Fahrenheit (37 degrees Celsius). Additionally, some enzymes work effectively at both high and low temperatures. For instance, while animals in desert environments have enzymes that can withstand higher temperatures, those in arctic environments have enzymes that can withstand lower ideal temperatures.

Because more substrate molecules can interact with the enzyme and make more product as a result, the reaction rate will quicken when more hydrogen peroxide is added to the mixture. More foam forms in your cup as you increase the H2O2 concentration in your reaction.

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gamma rays may be used to kill pathogens in ground beef. one irradiation facility uses a 60co source that has an activity of 1.0×106ci. 60co undergoes beta decay and then gives off two gamma rays, at 1.17 and 1.33 mev; typically 30% of this gamma-ray energy is absorbed by the meat. the dose required to kill all pathogens present in the beef is 4000 gy.

Answers

It is true that one irradiation facility uses a 60co source that has an activity of 1.0×106ci. 60co undergoes beta decay and then gives off two gamma rays, at 1.17 and 1.33 mev.

Irradiation facility is designed with overlapping layers of protection, interlocks, and safeguards to stop accidental radiation exposure. In addition, "melt-downs" don't occur in facilities as a result of the radiation supply provides off radiation and decay heat; but, the warmth isn't ample to soften any material.

A pathogen is outlined as an organism inflicting sickness to its host, with the severity of the sickness symptoms spoken as virulence. Pathogens are taxonomically wide numerous and comprise viruses and bacterium likewise as animate thing and cellular eukaryotes.

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A marine biologist and cancer researcher worked together to isolate the green fluorescent protein (GFP) gene from a sample of jellyfish DNA. Scientists have successfully inserted this gene into a cancerous tumor in humans in order for the tumor to glow so it can be more easily removed during surgery. What biotechnology is used to accomplish this procedure?

Gel electrophoresis
Polymerase chain reaction
Restriction enzyme digestion
Transgenic technology

A is incorrect

Answers

Answer:

The biotechnology used to accomplish this procedure is D transgenic technology.

Explanation:

Transgenic technology involves the introduction of a foreign gene into the genome of an organism, allowing the organism to express the traits encoded by the foreign gene. In this case, the marine biologist and cancer researcher isolated the GFP gene from jellyfish DNA and introduced it into the genome of a cancerous tumor in a human. This allowed the tumor to express the GFP protein, which made it glow and made it easier to remove during surgery. Transgenic technology is a powerful tool for studying gene function and has many applications in biotechnology and medicine.

Answer: D

Explanation:

I took the test

In pea plants, the allele for smooth pods (S) is dominant over the allele for constricted pods (s).
Which of the following pairs of terms are correctly matched?
Choose 1 answer:

Answers

Option d is correct because pea plants with the dominant S genotype have smooth and constricted pod phenotypes, which are controlled by the S gene.

What distinguishes a genotype from a phenotype?

A physical observation of a trait under the control of a gene is called a phenotype.

genotype, on the other hand, is a genetic observation that cannot be physically seen.

The dominant allele is the one that controls a plant's phenotypic characteristics; the other allele, which cannot express itself, is always present in a group, but only the dominant will manifest itself.

If the smooth allele is dominant over the other in a pea plant, it manifests in allelic combination and hides the expression of constricted pods.

SS is the genotype as a result, and the constricted pod is the

phenotype, so choice d is the appropriate one.

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Complete question is " In pea plants, the allele for smooth pods (S) is dominant over the allele for constricted pods (s).

Which of the following pairs of terms are correctly matched?

Choose 1 answer:

Ss - phenotype; smooth pod - genotype

SS - genotype; constricted pod - phenotype

SS - phenotype; constricted pod - genotype.

SS - genotype; constricted pod - phenotype".

cells synthesize a variety of complex molecules to include proteins, nucleic acids, lipids and carbohydrates. a large majority of the molecules that cells make are proteins. which three organelles are most involved in synthesizing proteins?

Answers

The three organelles that are most involved in protein synthesis include: Nucleus, Ribosomes and Endoplasmic reticulum (ER).

What is protein synthesis and its significance?

The protein synthesis is the formation of polypeptide chain from amino acids. It takes place in two stages of transcription and translation. Transcription is the transfer of genetic instructions from DNA to mRNA in the cell nucleus. It includes three steps: initiation, elongation, and termination. Translation is formation of protein from mRNA.

Proteins are largest, complex molecules that play many important roles in the body. They perform most of their work inside the cell and are necessary for the structure, function and regulation of body tissues and organs

What is needed for protein synthesis?

Several essential substances must be present for protein synthesis, including the 20 amino-acids that make up proteins. Another essential component is a set of enzymes that work in this process. DNA and another form of nucleic acid called ribonucleic acid (RNA) are also essential.

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5. Below is the DNA sequence for the orange tiger and the mutated DNA sequence of the
white tiger. Look at both sequences and explain
a. what type of mutation took place
b.
how you know.
DNA code
Mutated DNA code
CAG CAG CAG CAG CAG CAG
CAG CAG CAG TAG CAG CAG

Answers

The type of mutation that took place is the insertion of a nucleotide into the DNA.

What is mutation?

A major factor in the diversity of organisms is a mutation, which is a change in the genetic sequence. These alterations take place at a variety of levels and might have very varied effects. Mutations may have an impact on an organism by altering its phenotype, or they may have an impact on the way DNA codes the genetic information (genotype). When mutations take place, they may be completely fatal or they may result in the termination (death) of an organism. Mutated RNA code GTC GTC GTC ATC GTC GTCOrganisms having undesirable mutations do not live very long.

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PLEASE HURRY ITS TIMES

DNA carries the cell’s genetic information; however, it cannot leave the .

Because of this, it copies part of its nucleotide sequence into a complementary copy of

during the process called .

During this process, the enzyme,

separates the DNA strands.

One strand of DNA acts as the

from which nucleotides are assembled into a strand of RNA.

Each three letter sequence on the mRNA is called a .

The start codon is and the three stop codons are .

Once the mRNA leaves the nucleus it goes to the to prepare for protein

synthesis which is called .

During this process, a molecule brings the to the ribosome.

The amino acids are hooked together by bonds.

The tRNA is able to match up to the mRNA by the in its anticodons.

When the codon is reached, the chain is released into the .



Word Bank:
protein mRNA ribosome tRNA peptide amino acid complementary bases
helicase AUG template stop codon nucleus UAG, UGA, UAA translation cytoplasm transcription

Answers

DNA can’t leave the nucleus, copy of mRNA, transcription, helicase separates the DNA, one strand acts as the template, codon, AUG, stop codons are UAA/UAG/UGA, translation, mRNA goes to ribosome, peptide bonds, complementary bases, cytoplasm

Hope this is helpful and correct! This was the last unit I did so it’s not as fresh in my mind

in rna processing, the intervening coding sequence that is missing from the final mrna is called an .

Answers

Exons, which are protein-coding sequences, make up eukaryotic genes. Intervening sequences called introns, which may be crucial in gene regulation but are excised from the pre-mRNA during processing, are also present.

What coding sequence is still present in the finished mRNA?

After the final mRNA is created, the process of translation involves reading a succession of codons, which are three-base sequences. The Genetic Code, an RNA code, directs how codons are read.

The regions of coding are known as material exons, whereas the intervening non-coding sections are known as introns. The mature mRNA molecule is subsequently created by a procedure known as RNA splicing, which involves removing the introns.

Nucleic acid coding sequences refer to exons.

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