The two ways by which we can detect that gene editing have occurred in the bacteria are;
A) WESTERN BLOT; Here,the gene that is edited will not be able to synthesize the protein.With this,if we do western against the already edited protein,no band will eventually appear in the western blot,and this indicates/confers that the gene have been edited.
B) PCR FOLLOWED BY SEQUENCING; IN PCR,there is amplification of the region with the use of flanking end primers that is edited.
However,mean primers will actually be used in a way that one primer is upstream to site of editing and the other primer to the downstream.
From this stage,the PCR product that is amplified will be sent for sequencing,and this sequency result will further confirm if the gene has been edited or not edited.
Restriction enzyme can also be used if the place where the gene has been edited is known.
If actually the gene has been edited,the nucleotide sequence will get changed.Having to choose such enzyme that cut only one's wide type wild type DNA but not actually gene that is edited,the result will further be compared inorder to confirm whether the gene has been edited or not.
The two experiments that can be done to detect gene editing including
Western blot and PCR(polymerase chain reaction)
DNA contain all the genetic properties of a cell and comprises of nucleotides with unique protein(amino) sequences. Western blot helps to detect specific protein through the sequences from a protein sample and helps to detect if editing took place.
Polymerase chain reaction(PCR) helps in the amplification of DNA which
helps to verify where editing took place if any.
Read more on https://brainly.com/question/19340015
PLEASE HELP A.S.A.P!! WILL MARK BRAINLIST!! I ONLY HAVE 6 MINUTES!!
Karizma is surprising her mother with her favorite red carnations, but the flower shop only had white. So, Karizma places the white carnations in a solution of water and red food coloring. The carnations turned red by the next day. What could be the possible explanation?
A. Phototropism is responsible for pulling the food coloring from the roots to the petals.
B. The roots pulled up the food coloring with the water and it was transported through the phloem to the petals.
C. Gravity assisted the plant in carrying the food coloring to the petals.
D. Thigmotropism is responsible for pulling the food coloring from the roots to the petals.
E. The roots pulled up the food coloring with the water and it was transported through the xylem to the petals.
Answer:
The roots pulled up the food coloring with the water and it was transported through the xylem to the petals.
Explanation:
the xylem is one of the transport tissues in plants. It transfers water and nutrient from roots to stems and leaves.
What cell organelle is responsible for making the vesicles for exocytosis to occur?
Answer:
cell membrane i think
Explanation:
potatoes are high in carbohydrates?
Answer:
True
Potatoes are considered a healthy carb.
Hope this helps
Answer:
Yes they are high in carbohydrates because they are in fibre and it is considered a starch and a vegetable and a carb
Explanation:
Hope thus helps ❤♥️
To ensure that a human gene is translated properly in a bacterial cell, you should clone the _____________ for the gene instead of the genomic DNA fragment that contains the gene.
Answer:
complementary DNA (cDNA)
Explanation:
The complementary DNA (cDNA) is a region of DNA that is synthesized from an RNA template by using a reverse transcriptase enzyme. cDNA is usually used to clone genes from eukaryotic organisms in prokaryotes. cDNA cloning is a technique that consists of isolating and amplifying a self-replicating prokaryotic organism that includes in its genome a cDNA of interest. This technique enables the insertion of fragments of eukaryotic DNA (cDNA) into plasmids that subsequently are multiplied by cloning and finally sequenced.
How does the burning of fossil fuels and deforestation contribute to the overall amount of carbon dioxide?
Answer:
Forests store large amounts of carbon. When fossil fuels are burned, carbon from dead and decayed plants, animals and phytoplankton that lived hundreds of millions of years ago (before dinosaurs existed), is released into the atmosphere in the form of carbon dioxide. .
what is the difference between fibrous protein and globular protein
Answer:
Fibrous proteins are generally composed of long and narrow strands and have a structural role.
Globular proteins generally have a more compact and rounded shape and have functional roles.
Explanation:
fibrous proteins ARE something, whereas globular proteins DO something.
What is the complementary strand for UUC AGC CAG
Answer 21 please thanks
It is chromosomes.....
there are 23 pairs of chromosomes and 23+23=46
The autonomic nervous system carries messages to and from your
Eyes
Tounge
Ears
Heart
Hydrogen bonding allows for:
O adhesion
O cohesion
all of the answers are possible because of hydrogen bonds
O capillary action
Asbestosis is a condition that affects the
O A. heart
O B. brain
O C. lungs
D. kidneys
Answer:
lungs
explanation:
Asbestosis (as-bes-TOE-sis) is a chronic lung disease caused by inhaling asbestos fibers. Prolonged exposure to these fibers can cause lung tissue scarring and shortness of breath. Asbestosis symptoms can range from mild to severe, and usually don't appear until many years after continued exposure
Answer:
C.Asbestosis is long term inflammation and scarring of the lungs due to asbestos fibers. Symptoms may include shortness of breath, cough, wheezing, and chest tightness. Complications may include lung cancer, mesothelioma, and pulmonary heart disease. Asbestosis is caused by breathing in asbestos fibers.
PLEASE HELP, i’ll give you brainliest if it’s right please
Answer:
C
Explanation:
recessive traits can only be seen when there is no dominant trait present.
The mass of an element is the sum of the atomic number and the number of
A. electrons.
B. ions.
C. neutrons.
D. protons.
Answer:
a. electrons
Explanation:
The mass of an element is the sum of the atomic number and the number of protons.
What is atomic mass?Atomic mass is the total weight of all the particles (protons, neutrons, and electrons) in a single atom. It is expressed in atomic mass units (amu) or daltons (Da). The atomic mass of an element is determined by the number of protons and neutrons in the atomic nucleus.
Each proton and neutron has a mass of approximately 1 amu, and the atomic mass of an element is determined by the total number of these particles in the nucleus. The atomic mass of an element is unique and remains constant, regardless of the number of atoms of that element present. This value is important in determining the chemical properties of an element and the behavior of compounds and mixtures.
Learn more about atomic mass, here:
https://brainly.com/question/17067547
#SPJ6
LABEL THE FLOWER POSTER
Which of the following is true of nudibranchs but not of flatworms?
Nudibranchs possess a mixture of genes from both of their paren
Nudibranchs have half as much genetic material as their parents.
Nudibranchs are genetically identical to one another.
Nudibranchs are all the same color.
please hurry
Answer:Its d
Explanation:I took the test
If two 15N forces are acting on an object in different directions, what is the net force?
N
Answer:
The net force acting on an object is the combination of all of the individual forces acting on it. If two forces act on an object in opposite directions, the net force is the difference between the two forces.
Explanation:
Hope this helped
HELP ASAP:
how many trials did it take for half of the virtualium atoms to decay?
Answer:
Carbon-14 has a half-life of 5,730 years. Taking one atom of 14C, this will either have decayed after 5,730 years, or it will not. But if this experiment is repeated again and again, it will be seen that the atom decays within the half life 50% of the time.
Describe the pathway in a eukaryotic, somatic cell, from DNA to extracellularly secreted protein. Include detailed descriptions of each mechanism, process, location, molecules and potential outcome.
Answer: The pathway involves the transcription and translation processes.
Explanation:
The DNA present in the somatic cells of the eukaryotic organisms undergoes transcription in which the DNA is used as a template to form mRNA. It occurs in nucleus
The mRNA encodes for a amino acid chain for a specific protein in the process of translation. It occurs in cytoplasm and require a cell organelle called ribosome.
The amino acid sequence for different proteins are different.
Match the mineral categories to their best descriptions
Answer:
Halide minerals - Minerals that contain elements that combine with chlorine, fluorine, bromine, or iodine.
Rust - An example of a common oxide mineral.
Native Element Minerals - Contains atoms of a single element.
Sulfate minerals - Contains the elements sulfur and oxygen.
Explanation:
I just took the test and its correct. Hope you make a good grade!
The minerals categories matched to their best description are :
Halide minerals: Minerals that contain elements that combine with chlorine, fluorine, bromine, or iodine.Rust: An example of a common oxide mineral.Native Element Minerals: Contains atoms of a single element.Sulfate minerals: Contains the elements sulfur and oxygen.What are minerals?A mineral is a naturally occurring inorganic solid, with a definite chemical composition, and an ordered atomic arrangement.
Halide minerals are those that combine with halogens. Rust is a common oxide mineral. Sulfate minerals consist of sulfur and oxygen. Native minerals (originally found) are those that consist of only toms of a single element in pure form.
Therefore, the minerals are matched with their correct description.
To learn more about minerals, refer to the link:
https://brainly.com/question/89259
Regulated cell death that is a necessary part of development is called
Dis is mah little BaBy
Answer:
Awwwww
Explanation:
Soooooo cute!!!!!!! I hope you have a great day and i bet that dog gives great cuddles!!!!!!
1. How does pollution affect ecosystems? I
PLEASE HELP ILL GIVE 40 POINTS!!!
Which of the following statements is true?
Thunderstorms need cool, dry air to form.
Hurricanes have wind speeds under 74 miles per hour.
Thunderstorm clouds are tall and thick with flat bottoms.
all of the above
IT ALL OF THE ABOVE SIR HOPE THIS HELPS(☞ﾟヮﾟ)☞☜(ﾟヮﾟ☜)
MAY I GET BRINLIST
What is the Chemical solution for photosynthesis
Answer:
6 CO2+6H20==>6O2+C6 H12 06
light
Explanation:
why is freshwater beneficial to plants
Explanation:
freshwater beneficial to plants is because estuaries are usually shallow, sunlight can reach all levels of the water. Plants and algae are important to freshwater biomes because they provide oxygen through photosynthesis,
They enhance water quality and facilitate stop alga growth by victimisation nutrients created by fish waste, unconsumed food and organic scrap. They turn out element throughout daylight, that is employed by fish and helps stabilize pH scale. Fish, in turn, unleash unleash, that plants use as a food supply.
The development of retinoblastoma is associated with a mutation in the 13q14.2 region of the human genome. What is the principle of indexing different parts of the genome?
Answer:
Genome indexing is a strategy to find a pattern (i.e., a short sequence read) in a long text (i.e., the genome)
Explanation:
Genome indexing is a similar strategy used when a book is indexed. This strategy is used to find more rapidly a given sequence by allowing to align the problem sequence and thus approximate to the putative origin of the query sequence within the genome. Genome indexing enables to design PCR primers/probes, map probes at genome level, understand data obtained from gene expression analyses, etc.
Why would a cell use active transport to move materials? List 3 reasons.
help this is due in 30 minutes
Answer:
I think its D
Explanation:
B. The Sun is farthest south is the sky is true. A is wrong because in the Northern Hemisphere because the northern part of Earth is tilted most toward the sun. C is also wrong because in September and March – when the Sun shines directly on the Equator and the length of day and night is nearly equal not in winter. And D is wrong because South Pole has 24 hours of daylight not darkness.
AYUDAAAAAAAAAAA (si no sabes no respondas porfa)
Answer:
debajo
Explanation:
No entiendo la diagrama mucho pero pienso que estas son las respuestas:
el sol a plantas verdes- energía luminosa
plantas verdes son- organismos autotrofos
plantas verdes a animales- energía química
herbivoros a carnivoros son- organismos
las flechas cerca los animales- energía liberada heterotrofos y organismos saprófitos
If the age structure of a population is on the older side, birth rates tend
to be:
A.Higher than normal
B.Lower than normal
C.Has no effect