An inhibitor is added to an enzyme-catalyzed reaction at a concentration of 26.7 μM. The Vmax remains constant at 50.0 μM/s, but the KM increases by 150%. Calculate the Ki for this inhibitor.

Answers

Answer 1

Using the Michaelis-Menten equation competitive inhibition, the Inhibition constant, Ki of the inhibitor is 53.4 μM.

What is the Ki for the inhibitor?

The Ki of an inhibitor is known as the inhibition constant.

The inhibition is a competitive inhibition as the Vmax is unchanged but Km changes.

Using the Michaelis-Menten equation for inhibition:

Kma = Km/(1 + [I]/Ki)

Making Ki subject of the formula:

Ki = [I]/{(Kma/Km) - 1}

where:

Kma is the apparent Km due to inhibitor Km is the Km of the enzyme-catalyzed reaction[I] is the concentration of the inhibitor

Solving for Ki:

where

[I] = 26.7 μM

Km = 1.0

Kma = (150% × 1 ) + 1 = 2.5

Ki = 26.7 μM/{(2.5/1) - 1)

Ki = 53.4 μM

Therefore, the Inhibition constant, Ki of the inhibitor is 53.4 μM.

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Related Questions

HELP ME OUT PLS!!!!

What is the net force, to the nearest newton, that accelerates the ball?

A) 100 N

B) .000025 N

C) 40,000 N

D) 1000 N​

Answers

Answer:

100n

Explanation:

f= 0.05*2000=100 N

Describe the Principle of Continuity of State​

Answers

If any liquid is flowing in a pipe of cross-section area, then rate of flow of liquid across any cross-section remains constant.

Principle of Continuity of State​

According to the equation of continuity states that if any liquid is flowing in streamline flow in a pipe of non-uniform cross-section area, then rate of flow of liquid across any cross-section remains constant. In the Principle of Continuity of State​, Av = constant.

Where A refers to cross-sectional area and v is the velocity in which the fluid flows. It means

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What is the empirical formula for CH2OHCH2OH?

Answers

The empirical formula is the simplest way to express a compound.

Just add up all the number of atoms in that compound

So for this example it would be C2H4(OH)2 I think. Not entirely sure.

What happens to the pitch of a sound if the frequency is high?

Answers

The higher the frequency wave the higher the pitch of sound we hear

Someone can help me please??

Answers

C To D is ur answer

How to polymers work? ​

Answers

Answer:

A polymer is any of a class of natural or synthetic substances composed of very large molecules, called macromolecules, which are multiples of simpler chemical units.  Polymers make up many of the materials in living organisms and are the basis of many minerals.

Explanation:

Explanation:

The first man-made polymers were actually modified versions of these natural polymers. Celluloid, the stuff from which silent-movie film was made, was a plastic created from chemically modified cellulose. The first completely synthetic polymer (that is, made by people through chemical synthesis), invented in the early years of the twentieth century, was Bakelite: a plastic made by reacting phenol and formaldehyde under pressure at high temperatures.

# be careful #

Given 0.38 grams of N2, how many grams of NaN3 are needed

Answers

2 Fe2O3 + 3C 4Fe + 3CO2

what is the 3rd element on the periodic table?

Answers

The 3rd element would be Lithium

Answer:

Lithium

Explanation:

The 3rd (third) element on the periodic table is Lithium. Lithium is a soft, silvery, light alkali metal denoted with the symbol Li.

Water (2470 g ) is heated until it just begins to boil. If the water absorbs 5.47x105 J of heat in the process,
what was the initial temperature of the water?
Express your answer with the appropriate units.

Answers

Answer:

47.01 °C

Explanation:

Q = mcΔt

Mass of water = 2470g

c or specific heat capacity of water = 4.18 J °C g

Q = 5.47 x 10⁵ J, which is expanded 547,000 J

No idea what Δt is, but we do know the water began to boil, and we know that water's boiling point is 100°C, so the final temperature had to be 100°C.

547,000 J = (2470) x (4.18) x (Δt)

Rearrange equation for Δt

(547,000) / (2470 x 4.18) = Δt

Δt = 52.98°C

But we're not done. We're trying to find the INITIAL TEMPERATURE of the water, not the TEMPERATURE CHANGE. We already have the final temperature, which is 100°C, and now we have how much the initial temperature rose by to get to 100°C.

All that we have to do now is 100 - 52.98 = 47.02°C

The initial temperature of the water is 47.02°C

How many atoms are in 1mg of NaCl

Answers

The number of atoms in 1 mg of NaCl = 1 × 10^19 atoms

Calculation of quantity of atoms

Atomic mass of Na = 23

Atomic mass of Cl = 35.5

Therefore the molar mass of NaCl = 23 + 35.5 = 58.5g/mol

But 1 mg = 0.001g and 1 mol = 6.022 × 10^ 23

Therefore, If = 58.5g = 6.022 × 10^ 23 atoms

0.001g =X atoms

X atoms = 6.022 × 10^23 × 0.001/58.5

= 0.1 × 10^23 × 10^-³

= 1 × 10^19 atoms

Therefore, the number of atoms in 1 mg of NaCl =1 × 10^19 atoms.

Learn more about atoms here:

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What is the concentration of the following:

2.0mol of zinc sulphate in 10 dm^3 of solution

Answers

Answer:

Concentration is measured in moles per cubic decimetre: mol dm–3. To find the concentration of a solution, divide the number of moles by the volume in dm3. If the volume is in cm3, divide the volume by 1000 first to convert it into dm3.

Explanation:

June 1
June 8
June 11
5. What phase of the moon most likely will appear 28 days after phase C?
A Waning Gibbous
June 15
B
Waning Crescent
с
Waxing Crescent
D
Waxing Gibbous

Answers

Answer:

D

Explanation:

it's D because it goes waxing crescent to waxing gibbous

A student wants to test how plants grow in different
colors of light. Thinking about this experiment, what
are some of examples of each of the following? List at
least two in each.
DATA
VARIABLES
CONTROLS

Answers

Answer:

What is the question? I don't see it.

Fill in the blank to complete each statement
The land that supplies water to a river system is called a
Pollution from a single, identifiable source is called
Pollution that is difficult to link to a particular origin is called
source pollution
source pollution.

Answers

Answer:

1- Watershed

2- Point

3- Nonpoint

Explanation:

Just did it

Answer:

1. watershed

2. point

3. nonpoint

Explanation:

Calculate the value of K at 700 K for the
reaction
H2(g) + I2(g) → 2 HI(g)
if Kc = 54 at the same temperature.

Answers

Answer:

It going to be 54

Explanation:

I got it right but I would say it's because the temperature stays the same. So the K value stays the same.

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