A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid

Answers

Answer 1

Answer:

tetracycline

Explanation:

When the DNA insert is ligated in Pstl site, the ampicillin gene is disrupted while tetracycline gene is still intact.

- If the bacterial colony is growth in a medium with only ampicillin, only the cells that contains the plasmid with the insert will grow.

- If the bacterial colony is growth in a medium with only tetracycline, the cells which have incorporated the plasmid will survive. We cannot distinguish between cells with plasmid + insert and plasmid alone.

- If the bacterial colony is growth in a plate with ampicillin + tetracyclin, only the cells with the plasmid without the insert will grow.

Therefore, to ensure that the cells of a bacterial colony contains the plasmid, they have to be seeded in a plate with tetracycline.


Related Questions

The asthenosphere is the second layer of Earth characterized by

O soft rocks that flow easily
O layers of both hard and soft rocks
O magma
O hard rocks that break

Answers

Answer:

soft layers that flow easily.

the answer is soft rocks that flow easily

define the term cell division​

Answers

There are two types of cell division: mitosis and meiosis. Most of the time when people refer to “cell division,” they mean mitosis, the process of making new body cells. Meiosis is the type of cell division that creates egg and sperm cells. Mitosis is a fundamental process for life.

Answer:

please mark as brainliest

Explanation:

Cell division is the process by which a parent cell divides into two or more daughter cells.

The cell membrane (plasma membrane) is describe as a highly selective
barrier composed of alipid bi-layer. This structure plays an important role
in moving molecules across the cell membrane in order for the cell to
maintain homeostasis. If the cell is unable to take in the materials it needs
or remove vwhat it does not need, the cell may die. This inability of cells to
maintain homeostasis may cause sickness or even death of an organism.
Why is it important that the cell membrane is highly selective?
O So that the cell wall is stabilized and maintains its rigidity.
O To prevent certain ions and molecules from entering or exiting the celI.
O So that proteins and lipids can attach to the membrane where needed.
O To allow movement of materials in and out of the cel.

Answers

Answer:

O To prevent certain ions and molecules from entering or exiting the celI.

Explanation:

The cell needs to keep a constant chemical composition on the inside in order to mantain homeostasis

RIGHT ANSWER GETS BRAINLYEST!

The state of California experiences periodic occurrences of higher than average precipitation. This causes flooding due to heavy rainfall. Which weather phenomenon most likely causes this and why?


La Niña, because of weakening of trade winds

El Niño, because of weakening of trade winds

El Niño, because of strengthening of trade winds

La Niña, because of strengthening of trade winds


Which of these can be impacted due to climate?

Group of answer choices

Selection of crops

Seasonal crop yield

Aircraft visibility

Flight timing

Answers

strengthening of trade winds and selection of crops.

Predict the nature of the indicated covalent
bond.
H
H-C-N

H
H
H
polar
non-polar

Answers

Answer:

The nature of the indicated covalent bond is polar.

Explanation:

The given diagram is the Lewis structure of Methylamine which has the formula, CH₃NH₂.

In order to know that it is polar, we will discover that in the Lewis structure there are unpaired electrons in the central atom. Also, the bond that exists between the hydrogen and nitrogen shows that it is polar.

CH₃NH₂ is seen to have two charged ends that are opposite and the bonds that exists between hydrogen and nitrogen, which makes it a polar molecule.

The electronegativities of carbon, hydrogen and nitrogen can also be used to determine its polarity.

The forces shown in the diagram caused the rock layers to

A Fault

B Folohy

С Form

D Expand​

Answers

It would be very helpful if you could add the diagram

What is the term for an atom that decays?
A. Precambrian
B. Radioactive
C. Mesozoic
D. Sediment

Answers

Answer:

radioactive

Explanation:

its in physics term

Please I need help ASAP!!

Answers

Answer:

membrane becuase it filters what goes in and out of the cell

membrane :)) , seems more true bc those sent not

In animals, respiration takes place through cellular interaction using ...__ *

Answers

Answer:

Cellular respiration?

Explanation:

List where all of the ATP molecules come from and how you add up to the total

Answers

Answer:

Explanation:

ATP molecules are used by all living organism as energy to carry out life functions. Also notable, ATP stands for Adenosine Triphosphate. This molecule is composed of three parts:

Adenine

Ribose

Three Phosphate Groups



I’ll give brainlist if it’s outstanding

How do parachutes work?

Two paragraphs

Use all these terms in your paragraph: Gravity, Acceleration, terminal velocity, force, air resistance.



Help plssss

Answers

Answer:

Parachutes are made of light but strong materials to keep yyouin the air and have a safe landing :)

Explanation:

What are the harmful effects of pteridophytes?​

Answers

These species can spread rapidly with negative consequences for native species. Invasive plant species have an impact on the diversity of local species, they affect water availability and damage the quality of soil nutrients. Once an alien plant has invaded a habitat, it changes the conditions of that environment.

What is the resource in the plant that aphids eat?

Answers

Answer: Aphids are herbivores. They suck plant juices out of the leaves, stems, or roots of plants. The juices they drink often have much more sugar than protein. Aphids have to drink so much sugary juice to get enough protein that they excrete a lot of the sugar.

Explanation:

I can confirm that it is correct.^
Aphids feed on soft stems, branches, buds and fruits. They pierce the stems and suck the nutrient rich sap from the plant leaving damaged flowers and fruits :)

What is a pollinator?
an animal that transfers pollen from flower to flower
a colorful flower that attracts insects
an insect that makes pollen
a flower that produces fruit for animals to eat

Answers

Explanation:

an animal that transfers pollen from flower to flower

hope it helps!

An animal that transfers pollen from flower to flower

what are all of the effects of deforestation? Select ALL that apply

Answers

Answer:

Where are the answers?

Explanation:

A "healthy ecosystem" can also be described as:

Answers

Answer:

water and rocks; energy source, break down dead plants and animals, returning vital nutrients to the soil.

Explanation:

Choose TWO human activities that can help to increase the biodiversity of the Amazon rainforest
A
creating more urban settlements
B
mining and resource extraction
C
increasing agricultural run-off
D
practicing sustainable agriculture
E
creating protected land areas
TI
introducing non-native species

Answers

The answer would be A and C
Please mark me the brainliest ty

The two activities that can help to increase the biodiversity of the Amazon rainforest are as follows:

Creating more urban settlements.Increasing agricultural run-off.

Thus, the correct options for this question are A and C.

What is Biodiversity?

Biodiversity may be defined as the sum total of all the variety of living organisms that are present on the earth's surface. In a more simple sense, it is also characterized by the number of species of all living organisms exist on the earth.

Mining and resource extraction disrupts the quality and quantity of resources that living organisms utilize in order to enhance biodiversity. The introduction of non-native species utilizes all resources present in the area of native species and furnishes the process of competition. Creating protected land areas restricts the resources at specific parts/locations.

Therefore, the correct options for this question are A and C.

To learn more about Biodiversity, refer to the link:

https://brainly.com/question/11542363

#SPJ6

it's funni to be rog

Answers

Answer:

facts

Explanation:

kefka pfp go brr

Answer:

Ha

Explanation:

Mitosis produces which cell type?
haploid
diploid
chromosome
gamete

Answers

Answer:

b, diploid.

Explanation:

Mitosis produces two diploid somatic cells, genetically identical to each other and the parent cell.

Mitosis produces diploid cells (option B)

What is Mitosis?

Mitosis is a process of cell division observed in cells. Its primary purpose is to ensure that the genetic information, in the parent cell is equally distributed among the resulting daughter cells.

During mitosis the process generates cells that are diploid, meaning that the number of chromosomes in the parent cell is the same as the number in the resulting daughter cells.

In contrast haploid cells have half the number of chromosomes found in cells. Examples of haploid cells include sperm and eggs which are known as gametes.

Notably, we can conclude that mitosis produces diploid cells (option B)

Learn about Mitosis here https://brainly.com/question/1186551

#SPJ6

Please I need help will give the brainlist to the right answer

Answers

Answer:

if i’m not wrong i think it’s M?

Explanation:

the nucleus is like the boss of the cell so

how the body defends itself against infection

Answers

Answer: poop

Explanation: if you cover yourself in poop it heats your body up an animal does this but i cant remember which one

Answer:

Natural immunity: inborn defence. External defence is maintained by means of intact skin, by mucous, which traps and eliminates micro‐organisms, and by body secretions such as saliva and perspiration, which have disinfectant properties.

To grow, plant cells must produce new components of the cell membrane, including phospholipids and membrane proteins. Components of the cell membrane are most likely synthesized in which of the following cellular structures in a plant cell?

Answers

Answer:

endoplasmic reticulum

Explanation:

ER is full with proteins than help to synthesize phospholipids in plant cells

How might global climate change effect iguana populations?

Answers

Answer:

Since iguanas are cold blooded animals and can not regulate their own body temperatures, once temperatures reach 40° they become immobilized and they cant move, so if it is cold they will die.

Which of the features of this carbon-based macromolecule can be used to distinguish it from the other biological molecules?

Answers

Answer:

there are four major classes of biological macromolecules: 1. Proteins (polymers of amino acids) 2. Carbohydrates (polymers of sugars) 3. Lipids (polymers of lipid monomers) 4. Nucleic acids (DNA and RNA; polymers of nucleotides) Let’s take a closer look at the differences between the difference classes. Now that we’ve discussed the four major classes of biological macromolecules (carbohydrates, lipids, proteins, and nucleic acids).

Hopefully, this helps! And have a great rest of your day! :D

This question is incomplete. But I can answer Based on my previous knowledge.

The content of the Carbon-based macromolecules is the features that differentiate them from others.

The characteristics of a biomolecule are:

A chemical compound found in living organisms

They are formed by chemical substances composed mainly by:

CarbonHydrogenOxygenNitrogenSulfurPhosphorus

Biomolecules are the foundation of life and fulfill essential functions for living organisms. Additionally, Biomolecules can be:

Lipids, composed of Carbon (C), Hydrogen (H), Oxygen (O), and sometimes Phosphorus (P), Nitrogen (N), and Sulfur (S)Carbohydrates, composed of Carbon (C), Hydrogen (H), and oxygen (O)Proteins Carbon (C), Hydrogen (H), Oxygen (O), Nitrogen (N), Sulfur (S), and in some cases Phosphorus (P)Nucleic acids Carbon (C), Hydrogen (H), Oxygen (O), Nitrogen (N), and Phosphorus (P)

According to the above, each biomolecule can distinguish by the elements that compose it.

Learn more in: https://brainly.com/question/17962581

INEED Help thx :p with 5

Answers

the answer would be c

Gregor is planting pea plants in his backyard. Some of the plants are tall and the rest are short. Some of the plants are yellow and the rest are green. The numbers of different plant types are in the ratio[{Tall green}:{Tall yellow}:{Short green}:{Short yellow} = 8:5:2:1. If there are 1080 green plants, then how many total plants are in Gregor's yard?

Answers

Answer: 1732

Explanation:

infer short, yellow are recessive

8TG, 5Tg, 2tG, 1tg

proportion of green plants = 10/16

so total plants = 1080*16/10 = 1732

DONT ANSWER WITH THE SAMPLE RESPONSE ik I missplet that)


According to the author, what is the main reason for the decrease in trillium in the forest? What evidence does she give to support her claim?

Answers

The author cites a research study performed by scientists as evidence to support her claim. ... One was left open to deer, and the other was fenced off.

Answer:

The main reason for the decrease in trillium is the large number of deer in the forest that are eating the trillium. The author cites a research study performed by scientists as evidence to support her claim. In this study, researchers planted two fields, each containing garlic mustard and trillium. One was left open to deer, and the other was fenced off. Scientists kept track of the fields for several years. They found that trillium was able to regain much of its original space in the protected field. In the unprotected field, there was still more garlic mustard because the deer ate the trillium. The author also states that similar research studies have been conducted in many forests across the United States, and they all reveal similar results.

Explanation:

why do plants need to continually take in carbon dioxide, oxygen, and water from the environment?

Answers

Answer:

Oxygen is needed to break the sugar into carbon dioxide, releasing energy the plants can use to stay alive. However, plants also take in energy from the sunlight, carbon dioxide from the atmosphere, and water from the soil.

Explanation:

They use all of them to make sugar and release oxygen.

Hope this helps!

Brain-List?

Oxygen is needed to break the sugar into carbon dioxide, releasing energy the plants can use to stay alive. However, plants also take in energy from the sun(light), carbon dioxide from the atmosphere, and water from the soil; they use all of them in order to make sugar, and release oxygen.

Explain how each of the other fields of social science is important to historians. Remember they are economics, geography, archaeology, and political science. Give an example of how a historian would use each of these branches when studying history.

Answers

Answer:

Social science, any branch of academic study or science that deals with sociology, psychology, political science, and economics. Most historians, however, consider history as one of the humanities.

HOPES THIS HELPS BRO!!!

Which body system is responsible for motion in humans?
A
Immune
B
Integumentary
C
Muscular
D
Circulatory
.

Answers

Answer:

Muscular System.... off course

your answer is C. Immune system fights off viruses and stuff. Integumentary is your skin and Circulatory deals with your heart and oxygen :)
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