4. what experimental technique was utilized to show that dna replicated by a semiconservative process?

Answers

Answer 1

An experimental demonstration of semiconservative DNA replication was provided by the Meselson and Stahl Experiment.

What is Meselson and Stahl Experiment?An experimental demonstration of semiconservative DNA replication was provided by the Meselson and Stahl Experiment. In order to explore DNA replication, Matthew Meselson and Franklin Stahl carried out an experiment on the fast-diverging E. coli in 1958.In 1958, Matthew Meselson and Franklin Stahl performed an experiment on the rapidly diverging E. coli to investigate DNA replication. CsCl density gradient centrifugation was the method employed by Meselson and Stahl to explore semiconservative replication of DNA.Max Delbrück informed PNAS in May 1958 that the semiconservative method of DNA replication had been discovered by the well-known density labeling studies of Matthew Meselson and Franklin W. Stahl.

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Related Questions

2. what is the relative proportion of leukocytes in the blood at any given time? how about in the rest of the body fluids and tissues?

Answers

White blood leukocytes cell count is an indicator of health. 4,000 to 11,000 per μL of blood is a normal figure, or 1% of total adult blood volume. Blood cell production is then often regulated by the body structures such as the lymph nodes, spleen, liver, and kidneys.

Leukocytes A type of blood cell made in the bone marrow and found in the blood and lymphatic tissue. White blood cells are the part of the body's the immune system. They are help the body to fight infections and the other illnesses. the Leukocytosis means high white blood cell count. This means that you have more white blood cells than normal. Leukocytosis is a normal immune response and is not necessarily a cause for concern. In most cases, this means your body is fighting infection or inflammation.

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Where in the eukaryotic cell would you expect to find transcripts with intron sequences?.

Answers

Introns are found in the genes of most organisms and many viruses and can be present in both protein-coding genes and genes that function as RNA (non-coding genes).

protein-coding genes single gene or transcription unit can therefore encode multiple proteins or other gene products, depending on how the exons are joined. In fact, scientists estimate that there may be over 500,000 different human proteins, all encoded by just 20,000 protein-coding genes. According to the standard model, most RNA sequences are derived from protein-coding genes. That is, they way we and are processed into the messenger RNA (mRNA), exported to the cytosol, and then we translated into proteins.

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The process by which carbon is cycled between the atmosphere, land, water, and organisms.

Answers

The process by which carbon is cycled between the atmosphere, land, water, and organisms is called the carbon cycle.

Atmosphere is the mixture of gases present in any planet. These mixture of gases are said to be enveloping the whole planet which remains at its position to the gravitations force of that particular planet. The earth's atmosphere is composed of 78% nitrogen, 21% oxygen, and one percent other gases.

Carbon cycle is the flow of carbon between different living and non-living forms. Carbon is the most vital atom that makes up almost everything in the earth and hence it is necessary to maintain its cyclic flow and prevent it from being depleted to maintain the continuity of life.

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Besides recombination, what other event in meiosis increases the genetic diversity of the gametes?.

Answers

Answer:

Crossing over.

Explanation:

Crossing over is when the two sex cells or gametes exchange their genetic material during sexual reproduction to create 2 UNIDENTICAL daughter cells.

Unidentical daughter cells = genetic variation because they are not identical.

when azt, a dideoxynucleotide was added to cancer cells, the dna replication stopped and cancer cells stopped proliferation. during which stage of such inhibition would happen?

Answers

DNA replication and cancer cell proliferation both came to an end during S phase.

How can cancer be found?

Weight fluctuations, such as unanticipated growth or reduction. Skin changes like redness, yellowing, or darkening, as well as open wounds that aren't healing or changes to existing moles. changes to the bladder or bowel habits. breathing problems or a persistent cough.

What kind of cancer has the highest mortality rate worldwide?

Roche | Lung cancer is the most fatal cancer in the world. More people die from lung cancer each year than from breast, colorectal, & prostate cancers put together. 1 Despite the recent surge of novel, life-extending medications, lung cancer still has a high mortality rate.

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Based on your knowledge of microbial genetics and microbial pathogenesis, where do you think the genetic information for endotoxins are located?.

Answers

The genetic information of endotoxin is located in the chromosomes.

Endotoxins are the type of toxins present inside the bacterial cell. They are also known by the name pyrogens and they ruin the immune system. The location of endotoxins is the cell wall of Gram-negative bacteria. The nature of endotoxins is lipopolysaccharide.

Chromosomes are the compact form of DNA. These are comprised of DNA and histone proteins that are bound together in a highly packed form. The genetic material is transferred from parent to offspring in this compacted chromosomal form. Each chromosome is made of two sister chromatids which are joined together at the site called centromere.

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which amino acid would you expect to be part of an alpha-helix from the gpcr signaling protein? a. leucine b. asparagine c. tyrosine d. glutamic acid

Answers

The amino acid to be part of an alpha-helix from the gpcr signaling protein is leucine.

One of the three necessary branched-chain amino acids is leucine (BCAAs). Skeletal muscle can utilise these amino acids to provide energy during exercise.

Consuming foods with complete proteins provides adequate amounts of these amino acids. Foods including meat, poultry, fish, eggs, and milk fall under this category. Studies have not consistently demonstrated that consuming supplements of these amino acids enhances exercise performance, increases muscle mass, or aids in exercise recovery.

Leucine may aid in bone and skin repair. Lean body mass and muscle growth could both be accelerated. It might boost human growth hormone production (HGH). It might aid in blood sugar regulation.

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when organisms with certain heritable traits have an increased chance of survival and leave more viable offspring in a particular environment, then what evolutionary force is operating?

Answers

Natural selection is the evolutionary operating force when organisms with certain heritable traits have an increased chance of survival and leave more viable offspring in a particular environment.

Natural selection refers to a non-random variation in reproductive output among replicating entities, frequently caused indirectly by variations in survival in a particular environment, which results in an increase in the proportion of advantageous, heritable traits within a population from one generation to the next. Natural selection is the consequence of the interaction of a few critical ecological and genetic factors.

As in the case of the development of antibiotic and pesticide resistance or the effects of excessive human predation, the situations in which such conditions occur are frequently of direct consequence to human health and well-being. One of the main mechanisms behind evolutionary change is natural selection, which is how adaptive traits evolve.

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Explain the concepts of structure and function. How might structure and function relate to biology (the study of living things)?


Please give me an explanation!! Will mark as brainliest !!!! This will be worth 10 points!!!!

Answers

Structure dictates function is one among biology's core ideas; how something is put together enables it to carry out a certain purpose. This is often seen at every level of the biological organization hierarchy, from atoms to the biosphere.

The link between structure and performance is what allows all living things and living systems, from molecules to organisms, to function successfully.

What about structure and function?Numerous fields of science and engineering share the thought of structure and function, sometimes referred to as form and function.Scientists might want to understand how an organism's structure or the composition of a substance affects how it functions or behaves.Then, a spread of other phenomena may be explained by this underlying physical link (a relationship between structure and function).For instance, the body's metabolic requirements must be satisfied by the flow of oxygen and CO2 through the tissues and into the lungs.Load-bearing structures include things like buildings, skeletons, anthills, beaver dams, and salt domes.The infrastructure of a person's society is made up of buildings and non-building structures that are the outcome of construction.Production is simpler and of higher quality when workers with similar abilities and experiences are grouped together.There is little time spent acquiring new skills because roles and responsibilities don't vary often, and responsibility is clear .

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what is the third difference that you have learned about in this lesson?

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The third difference between plants and animals from the lessons learnt so far in this class is that:

Plants can manufacture their food by themselves while animals cannot but depend on organisms for food.

Why plants can make food on their own and animals cannot

Plants are able to synthesize their food by themselves through photosynthesis due to the presence of chlorophyll in their chloroplasts. Animals cannot produce food on their own simply because they lack the green pigment chlorophyll and so, they depend on other organisms for food.

In conclusion, it can be deduced that plants are autotrophs while animals are heterotrophs.

Complete question:

Biology/ characteristics of living organisms/differences between plants and animals.

What is the third difference that you have learned about in this lesson between plants and animals.

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Describe how the two tools of molecular biology—often described as scissors and glue—are used in conjunction with plasmids to make copies of a gene of interest.

Answers

Restriction enzymes act as molecular scissors producing cuts at or near sequence specific regions (i.e, restriction sites) at both the strands of a double stranded DNA molecule. On the other hand, DNA ligase enzymes act as molecular glue and help in joining the fragmented DNA pieces together by forming phosphodiester bonds.

What are restriction enzymes?

Restriction enzymes are naturally found in bacterial and archaebacterial cells. Such enzymes protect these unicellular cells by cleaving and destroying foreign genetic particles injected by bacteriophages (i.e, viral molecules responsible for infecting bacterial cells). Classically, there are four types of restriction enzymes i.e, types I, II, III and IV. Example: EcoRI, EcoRII, HindIII, etc.

What is DNA ligase?

DNA ligase, a specific kind of enzyme helps in connecting fragmented DNA strands by facilitating the creation of a phosphodiester bond. It helps the cells to repair single-strand breaks in their duplex DNA, while other varieties (like DNA ligase IV) may only repair double-strand breaks (i.e. a break in both complementary strands of DNA). DNA ligase uses the corresponding strand of the double helix as a template to repair single-strand breaks, producing the last phosphodiester bond necessary to finish the repair of the DNA. Thus, both DNA replication and DNA repair need DNA ligase. Example: T4 DNA ligase, E.coli DNA ligase, etc.

What is molecular cloning?

In this process, a particular gene of interest or the insert can be introduced into a plasmid vector by producing a cut at both the vector and the gene with the same enzyme thus, producing sticky ends and ligating the two fragmented pieces together to produce a recombinant product.

What is the role of the restriction enzymes in molecular cloning?

The isolated DNA has to be incubated with the specific restriction enzymes under optimal conditions.This helps the restriction enzymes in producing sequence specific cuts at specific restriction sites in the DNA molecules. This catalytic reaction is called restriction digestion.Agarose Gel Electrophoresis helps in analyzing the progression of the restriction digestion reactions.On the other hand, the plasmid vector DNA also has to be processed using similar procedures.

What is the role of the DNA ligase enzymes in molecular cloning?

Ligation is the process of fusing the two DNA fragments together with the enzyme, DNA ligase.The interest molecule and the vector are combined to create the final DNA molecule. As a result, the new hybrid DNA molecule is also referred to as a recombinant DNA molecule, and the technique is known as recombinant DNA technology.

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Restriction enzymes are considered as scissors that cleave DNA into fragments at or near specific recognition sites. DNA ligases act as a glue that helps in joining the DNA strands together by catalyzing the formation of a phosphodiester bond.

What are restriction enzymes?

These are protein isolated from bacteria that cleaves DNA sequences at sequence-specific sites, producing DNA fragments with a known sequence at each end. It helps in recombinant DNA technology and genetic engineering.

What are the DNA ligases?

It is a specific type of enzyme that plays a role in, maintaining genomic integrity by repairing single-strand breaks in duplex DNA in living organisms.

In the gene cloning process, Both the plasmid and the DNA sequence of interest are cut with restriction enzymes to generate compatible overhang sticky ends that allow them to bind. The enzyme DNA ligase joins the fragments with matching ends together to form a single, unbroken molecule of DNA. This produces a recombinant plasmid that contains the target gene.

Hence restriction enzymes act as molecular scissors and ligases act as a molecular glue.

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Which of the following statements regarding fecal transplant is correct?

a. Donors and recipients must be carefully matched to prevent an immune reaction in the recipient
b. Fecal matter must be carefully disinfected and screened for viruses, cysts, and endospores before transfer to a recipient patient.
c. This treatment is designed to jump-start the intestinal microbiota after an infection with Clostridium difficile resuts in acute diarrhea.
d. The goal is to completely eradicate Clostridium difficile from a patient's gut microbiota and replace it with a healthy community

Answers

Option B is correct Fecal matter must be carefully disinfected and screened for viruses, cysts, and endospores before transfer to a recipient patient.

Fecal transplantation is the process of removing feces, often known as stool or poop, from a healthy donor and placing them into the digestive system of a patient. By introducing beneficial bacteria into the recipient's intestines, the technique can control the infection Clostridium difficile, or C. diff. Both adults and children can undergo FMT. The restoration of beneficial bacteria via fecal transplant has been shown to help control C. diff and prevent its recurrence. FMT has the potential to control C. diff more reliably than antibiotics in several situations.

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Which of the following is a reason that argues against clear-cutting a patch of a tropical rainforest ecosystem? (Specify all that apply.)
We have not yet learned about all the species that exist in the ecosystem that could be useful for humans.
It will change the soil microbiome in ways that prevent regrowth.
It may result in the extinction of animals in the food web.
The trees are important for storing carbon.

Answers

The reason that argues against clear-cutting a patch of a tropical rainforest ecosystem include the following below:

It will change the soil microbiome in ways that prevent regrowth.It may result in the extinction of animals in the food web.

What is an Ecosystem?

This is a term which is referred to as the combination of of all the organisms and the physical environment with which they interact.

Clear-cutting a patch of a tropical rainforest ecosystem will lead to a change in the soil microbiome which may prevent regrowth and may lead to extinction of some animals as a result of their habitats being destroyed thereby decreasing their survival rate.

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How is the energy produced by photosynthesis stored?

Answers

Answer:

This transforms the water into oxygen and the carbon dioxide into glucose. The plant then releases the oxygen back into the air, and stores energy within the glucose molecules. Inside the plant cell are small organelles called chloroplasts, which store the energy of sunlight.

Energy produced by photosynthesis is stored inside the glucose molecules(i.e, sugars).

What is photosynthesis?

It is a process in which green plants and other autotrophic organisms make use of sunlight, water and carbon dioxide to synthesize nutrients. They store energy in the form of sugars. Oxygen is generated as a by-product of photosynthesis, and it involves the use of the green pigment chlorophyll.

Algae also make use of photosynthesis to convert solar energy to chemical energy. Oxygen is once again a by-product of this reaction.

Photosynthesis occurs in all plants and blue-green algae. It takes place in all green parts of a plant including its leaves, stems, sepals etc.

Hence, the energy produced as a result of photosynthesis is stored in organic molecules (glucose, i.e, sugars).

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What is the role of epinephrine in cell signaling?

Answers

Epinephrine plays a crucial role in the fight-or-flight response as a cell signaling chemical.

What is cell signaling?

Chemical signals are frequently used by cells to communicate. These chemical signals, which are proteins or other compounds made by the transmitting cell and released into the extracellular space, are frequently secreted by the cell.

Role of epinephrine in cell signaling:

An essential cell signaling molecule in the fight-or-flight response is epinephrine. Epinephrine, also referred to as adrenaline, is a powerful messenger that signals various cell types throughout the body with a variety of effects. Epinephrine binds to receptors on smooth muscle cells that surround the bronchioles in the lungs. As a result of the muscles relaxing, the bronchioles widen, allowing more oxygen to enter the bloodstream. Epinephrine causes the pacemaker cells of the sino-atrial node of the heart to beat more quickly. This speeds up the flow of oxygen, glucose, and other chemical signals to the cells that require them. Additionally, epinephrine constricts particular subcutaneous muscle cells, resulting in beads of sweat and raised hairs on the skin's surface.

Hence epinephrine plays a crucial role in cell signaling.

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which of the following is a hypothesized benefit of foam roller exercises? 1. increased overlap of the sarcomeres within muscle 2. restored water balance in the muscles and fascia 3. significant reduction in scar tissue 4. increased protective tension in fascial tissues

Answers

The hypothesised benefit of foam roller exercises is option 4: increased protective tension in fascial tissues.

By releasing adhesions in your muscles and connective tissue known as myofascial buildup, using a foam roller can help reduce muscle tenderness. Foam rolling can improve blood flow by exerting pressure on specific points on muscles, tendons, and ligaments to loosen tight soft tissue.

Foam rolling is said to reduce muscle soreness and tiredness while also increasing muscular performance and flexibility. When using a foam roller, athletes exert pressure to the soft tissues while rolling, whereas when using a roller massager, the pressure is applied to the target muscles with the upper extremities.

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You have a new lens with a power of 60x and are excited to look at bacterial cells under one of our microscopes with this new lens. You screw it onto the nosepiece and look through the eyepiece, what is the total magnification of the bacterial cells?.

Answers

The total magnification of the bacterial cells when the power of the new lens is 60x is 600x.

 Power of eyepiece ×  power of the objective lenses =The total magnification

10X × 60X=600X

Normally the eyepiece magnification power is given as 10x . The common ocular magnifies ten times, marked as 10x. The standard objective lenses magnify 4x, 10x and 40x. If the microscope has a fourth objective lens, the magnification will most likely be 100x but for our case, I have worked with 10x. Therefore the total magnification will be 600x for the bacterial cells, looking at bacterial cells under one of our microscopes with this new lens.

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Which sentence is the best example of a living thing obtaining matter and
energy?
OA. A sea anemone moves its tentacles in response to touch.
B. An African violet plant grows from a leaf cut from an existing
plant.
C. A tadpole increases in size as its cells divide into many more cells.
D. An oak tree absorbs sunlight and carbon dioxide through its
leaves.

Answers

I believe D would be the most correct

Answer: D. An oak tree absorbs sunlight and carbon dioxide through its leaves.

Explanation: verified

Which statement explains why the tetra fish are able to produce a green glow?
A.
The tetra fish were bred with jellyfish, which produced offspring that can make the protein for green light.
B.
The genetic material of both the jellyfish and tetra fish were combined, which created a fish with all traits from both species.
C.
The inserted gene gave the tetra fish the ability to make the protein that produces green light.
D.
The protein that produces green light was taken from the jellyfish and injected into the tetra fish.

Answers

Answer:b maybe

Explanation:

Which of the following is not a kingdom?

A) Fungi

B) Protista

C) Mammals

D) Plants

Answers

Answer:

The answer is C.

Explanation:

Which events occurs during interphase?

Answers

Answer:

Explanation:, the cell grows and the nuclear DNA is duplicated

Answer: Three events occur during interphase: G1 phase, S (synthesis) phase and G2 phase.

21. Energy is the ability to
gain power.
do work.
measure electricity.
apply heat.

Answers

Your answer is to gain power
Energy is the ability to do work

Why are soft-bodied organisms less likely to leave a fossil trace than hard-bodied organisms?.

Answers

Proteins  are soft-bodied organisms less likely to leave a fossil trace than hard-bodied organisms.

soft-bodied organisms are much more likely to decay than organisms with difficult body elements along with bones, shells or enamel. as a consequence the fossil document consists of many stays of creatures like fish, clams and mammals, and relatively few stays of creatures like jellyfish, earthworms or wild flora.

Animals with difficult shells and skeletons are represented nicely within the fossil record. alternatively, soft-bodied animals are probable represented very poorly. it is possibly that maximum gentle-bodied species that ever existed are long gone all the time with out a trace. Land animals are probable very poorly represented as nicely.

The maximum not unusual approach of fossilisation is petrification through a system called permineralisation. After a shell, bone or enamel is buried in sediment, it is able to be exposed to mineral-rich fluids moving via the porous rock fabric and turns into filled with preserving minerals consisting of calcium carbonate or silica.

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why do ecologists ask questions about events and organisms that range in complexity from an individual to the biosphere

Answers

Ecologists investigate events and animals ranging by complexity from either a specific individual to the whole biosphere in order to comprehend linkages within the biosphere.

What is biosphere ?

All ecosystems on Earth that support life are collectively referred to as the biosphere. The biosphere includes everything, including the deepest tree roots, the shadowy oceanic depths, lush rain forests, majestic mountain summit, or transitional zones like this one marine and terrestrial ecosystems converge. The biosphere, usually referred to as the ecosphere, seems to be the totality of all ecosystems on the planet. A "life zone" surrounding Earth is another name for it. With few inputs and outputs, the biosphere is essentially a closed loop with respect to matter.

What are the three example of biosphere and its types ?

The biosphere, which includes both soil, water, and air, is the area of the planet wherein life can be found. These three parts are known as that of the lithosphere, hydrosphere, or atmosphere. Its types include: The vast biosphere is split up into various biomes. Scientists divided biomes into five categories: aquatic, tundra, grassland, woodlands, and deserts.

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the three important ideas mendel postulated concerning the transmission of traits from parents to offspring include:

Answers

The three essential thoughts Mendel postulated regarding the transmission of traits from mother and father to offspring include:

1. An character carries two variations of genes in step with the trait.

2. the two alleles of a gene separate during gamete formation so that every gamete receives a single allele in keeping with the trait.

3. tendencies exist in two paperwork -dominant and recessive.

He made 3 key choices approximately his experiments that played a crucial role in the development of his legal guidelines of inheritance management over breeding, use of purebred flowers, and statement of “both-or” tendencies that appeared in the simplest trade bureaucracy.

The important thing principles of Mendelian inheritance are summed up with the aid of Mendel's three legal guidelines: the regulation of independent assortment, the law of Dominance.

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how to calculate partial pressure

Answers

Use PV=nRT to calculate the individual pressure of each gas in a mixture

please help i’m stuck once again

Answers

Answer:

Gringo

Explanation:

If someone is uncomfortable identifying with the gender normally associated with their biological sex, then he could be classified as experiencing ________.

homosexuality
bisexuality
heterosexuality
gender dysphoria

Answers

If someone is uncomfortable identifying with the gender normally associated with their biological sex, then the person could be classified as experiencing gender dysphoria.

Gender dysphoria is the mental state of an individual where he or she feels uneasy or discomfort in accepting or even understanding the mismatch between their biological sex and their gender identity. This discomfort can cause other symptoms like stress, anxiety, depression, etc.

Anxiety is the psychological condition where the person feels restless and uneasy due to some reason that may not actually be very worrying. The other conditions accompanying anxiety are:  Fast heart rate, rapid breathing, sweating, etc.

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using the information from the graph, which of the following would be the most effective strategy for supporting high bee diversity?

Answers

Don't forget to convert from maize to switchgrass and add strips of the prairie to the information from the graph.

This question asks us what issue is the maximum possible to have caused the trade in the range of hen species that happened between years 30 and 60. The growing wide variety of chook habitats is one of the maximum contributing factors to the exchange within the range of bird species.

The common temperature for each month is plotted on the graph with a red dot and the dots are then linked in an easy, red line. Precipitation (mm) is measured by the usage of the numbers at the proper hand aspect of the chart.

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explain why the amplification of surface area is important to the function of chloroplasts and mitochondria.

Answers

To have the power to convert carbohydrates into ATP, chloroplasts require an increase in surface area.

What are the mitochondria's primary roles?

Definition. The majority of the chemical energy energy to operate the cell's biochemical reactions is produced by mitochondria, which are membrane-bound cell organelles. Adenosine triphosphate, a little molecule, serves as a storage space for the chemical energy generated by the mitochondria.

Where can you find mitochondria?

Cellular organelles called mitochondria transform the energy from the food into a state that can be utilized by cells. Numerous mitochondria, which are found in the liquid surrounding the nucleus of each cell, number in the hundreds to thousands.

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